口腔疾病防治2017，Vol.25Issue(11)：687-692,6.DOI:10.12016/j.issn.2096-1456.2017.11.002
牙龈卟啉单胞菌脂多糖对高脂血症兔腹腔巨噬细胞炎症相关因子基因表达的影响
Effect of Porphyromonas gingivalis lipopolysaccharide on gene expression of inflammatory cytokines in peritoneal macrophages of hyperlipidemic rabbits
摘要
Abstract
Objective To compare the inflammatory reaction of peritoneal macrophage after Pg-LPS stimulated in healthy rabbit and hyperlipidemia rabbit. Methods 12 New Zealand rabbits were randomly divided into 2 groups with 6 rabbits in each group, and normal diet and high-fat diet were fed to them respectively. The hyperlipidemia model was set up after 6 weeks. The peritoneal macrophage in normal and hyperlipidemia group were isolated and cultured, and then the cells in both groups were respectively divided into 3 groups:control, 1μg/mL Pg-LPS, and 1μg/mL E.coli-LPS. After 24 h treatment, the expressions of C-reaction protein (CRP), interleukin-1β (IL-1β), interleukin-6 (IL-6) , interleukin-8 (IL-8), and tumor necrosis factor-α(TNF-α) were detected by realtime PCR. Results The levels of CRP, IL-1β, IL-6, IL-8, and TNF-αwere higher in hyperlipidemia control group than normal control group. The expressions of inflammatory substances were increased after stimulated by Pg-LPS, and statistically higher in hyperlipidemia rabbit group than normal group (P<0.05). Conclusion Pg-LPS can enhance the mRNA expressions of inflammation related factors in peritoneal macrophage in hyperlipidemia rabbit.关键词
牙龈卟啉单胞菌/脂多糖/高脂血症/腹腔巨噬细胞/炎症因子/牙周炎Key words
Porphyromonas gingivalis/Lipopolysaccharide/Hyperlipidemia/Peritoneal macrophage/Inflam-mation factor/Periodontitis分类
医药卫生引用本文复制引用
游晓庆,骆凯,李艳芬,吴春芳,闫福华..牙龈卟啉单胞菌脂多糖对高脂血症兔腹腔巨噬细胞炎症相关因子基因表达的影响[J].口腔疾病防治,2017,25(11):687-692,6.基金项目
国家自然科学基金(81371152,30973326) （81371152,30973326）
福建省自然科学基金项目(2013J01306) （2013J01306）
