广西师范大学学报（自然科学版）2017，Vol.35Issue(4)：91-97,7.DOI:10.16088/j.issn.1001-6600.2017.04.013
多肽探针结合纳米银催化反应-吸收测定H CG
Peptide Probe Combined Nanosilver Catalytic Reaction-Surface Plasmon Resonance Spectrophotometric Detection for Trace Human Chorionic Gonadotropin
摘要
Abstract
In pH 7.4 Na2 HPO4-NaH2 PO4 buffer solution with the presence of human chorionic gonadotropin peptide (HCGP ) probe, the AgNPs can be aggregated to bigger AgNPs clusters. However,AgNPs can be well dispersed into this solution when the human chorionic gonadotropin (HCG)is added to form stable HCGP-HCG composite.The well-dispersed AgNPs exhibits catalysis on the reaction of H2 O2 reducting HAuCl4 into AuNPs which appeares a strong surface plasmon resonance (SPR)peak at 550 nm.With the increase of HCG concentration,the SPR peak increases linearly at 550 nm.The increased SPR absorption (ΔA550 nm)is linear to HCG in the range of 0.25~50μg/L,with a regression equation ofΔA550 nm=0.0124C+0.02,a correlation coefficient of 0.9878,and a detection limit of 0.2 μg/L.Thus,a simple,low-cost and sensitive nanosilver-catalytic SPR spectrometry was developed for the determination of HCG.关键词
多肽探针/人绒毛膜促性腺激素/AgNPs/纳米催化/SPR光度法Key words
peptide probe/human chorionic gonadotropin/AgNPs/nanocatalysis/SPR absorption分类
化学化工引用本文复制引用
李重宁,潘宏程,刘庆业,梁爱惠,蒋治良..多肽探针结合纳米银催化反应-吸收测定H CG[J].广西师范大学学报（自然科学版）,2017,35(4):91-97,7.基金项目
国家自然科学基金(21667006,21567005,21465006,21477025,21367005 ) （21667006,21567005,21465006,21477025,21367005 ）
广 西 自 然 科 学 基 金(2013GXNSFFA019003) （2013GXNSFFA019003）
