广西植物2017，Vol.37Issue(11)：1368-1377,10.DOI:10.11931/guihaia.gxzw201612020
蓝亚麻花瓣中类黄酮化合物及代谢途径分析
Flavonoid related compounds and their biosynthesis pathways in Linum pernne
摘要
Abstract
Flower colour is one of the most ornamental quality in ornamental plant, and flavonoid is the main pigment. In this study, the flowering stages of Linum pernne was divided into five stages. We used high performance liquid chromatography with a photodiode array detector(HPLC-PAD) and HPLC-electrospray ionization-mass spectrometry(HPLC-ESI-MS) method for qualitative and quantitative analyses of anthocyanins and the key flavonoid intermediate product in the different flower development stages. The results were as follows: L. pernne petals contained delphinidin, cyanidin and malvidin, but pelargonidin was no detected, and the main color substance was acylation of delphinidin; The highest total contents of the anthocyanins was at Stage 2. According to the end product and the intermediate product, we speculate the flavonoid biosynthesis pathways in L. pernne, in which F3′5′H leading branch pathway is dominant, and the main reason for that is F3′5′H enzyme activity is higher than that of F3′H.关键词
蓝亚麻/花青素苷/类黄酮/花色/代谢途径Key words
Linum pernne/anthocyanin/flavonoid/flower colour/biosynthesis pathways分类
农业科技引用本文复制引用
宋洋,雷霆,金雪花,付灯祥..蓝亚麻花瓣中类黄酮化合物及代谢途径分析[J].广西植物,2017,37(11):1368-1377,10.基金项目
国家自然科学基金(31560568),云南省人才培养基金(KKSY201323131)[ Supported by the National Natural Science Foundation of China(31560568) （31560568）
Program Training Talents in Yunnan Province(KKSY201323131)]. （KKSY201323131）
