广西植物2017，Vol.37Issue(11)：1378-1385,8.DOI:10.11931/guihaia.gxzw201706027
皂荚种质资源SCoT遗传多样性分析及指纹图谱的构建
Genetic diversity and fingerprints of Gleditsia sinensis germplasm resource based on SCoT
摘要
Abstract
Genetic diversity of eighteen Gleditsia sinensis germplasms was analyzed based on SCoT markers, the cluster analysis on eighteen G. sinensis germplasms was carried out by UPGMA method, and the SCoT fingerprints of eighteen G. sinensis germplasms were constructed by the polymorphic bands. The results of amplification showed that, 226 SCoT bands were obtained from fifteen primers selected from 51 SCoT primers, including 216 polymorphic bands, with a polymorphism rate of 96.61%. The average of the polymorphism information content( PIC), observed number of alleles (Na), effective number of alleles(Ne), Nei's gene diversity (H) and Shannon's information index (I) were 0.8759, 1.9649, 1.4401, 0.2726 and 0.4261, respectively. The genetic similarity coefficients of these samples were between 0.4914 and 0.9381. Therefore, it indicates that there are rich genetic diversities among these materials. The results of cluster analysis showed that, eighteen G. sinensis germplasms could be divided into three groups when the genetic similarity coefficient was 0.60. G. heterophylla formed the first group, G. melanacantha and Zaojia-T formed the second group, and the others formed the third group. DNA fingerprints of eighteen samples were constructed from eight polymorphic loci amplified by three primers, and these materials could be distinguished and identified accurately. All these results provide the important information for the identification and breeding for new cultivars of G. sinensis.关键词
皂荚/SCoT/DNA指纹图谱/遗传多样性Key words
Gleditsia sinensis/SCoT/DNA fingerprint/genetic diversity分类
农业科技引用本文复制引用
张安世,骆扬,范定臣,张中海..皂荚种质资源SCoT遗传多样性分析及指纹图谱的构建[J].广西植物,2017,37(11):1378-1385,8.基金项目
河南省科技攻关项目(14210221100850) （14210221100850）
2015年河南省林业厅科技兴林项目[Supported by Science and Technology Pro-gram of Henan(14210221100850) （14210221100850）
Program of Forest Initiated by Technology from Forestry Department of Henan Province in 2015]. （）
