广西植物2017，Vol.37Issue(11)：1395-1405,11.DOI:10.11931/guihaia.gxzw201701005
红花草莓的组织培养与快繁技术研究
Tissue culture and rapid propagation technique of red-flowered strawberry
摘要
Abstract
We used leaves of red-flowered strawberry as materials, by selecting the best medium for the callus induction, adventitious bud induction and root growth to establish a rapid propagation technique system of red-flowered strawberry. The results showed that TDZ was better than 6-BA in the induction of callus, and TDZ and NAA combination had better effects than TDZ and IBA. When the concentration of 6-BA was 0.5 mg·L-1, the inductivity of adventitious bud reached 86.6%. The low 6-BA concentration and 8 g·L-1 agar were benefical for seedling growth. NAA was better than IBA in the rooting inducing. So the optimum medium for callus inducing was: MS + 1.0 mg·L-1 TDZ + 0.5 mg· L-1 NAA + 30 g·L-1 sucrose +7 g·L-1 agar; the optimum medium for adventitious bud inducing was MS + 0.5 mg·L-16-BA + 0.1 mg·L-1 NAA + 30 g·L-1 sucrose + 7 g·L-1 agar; the optimum medium for seedling growthing was MS +0.1 mg·L-16-BA + 0.1 mg·L-1NAA + 30 g·L-1 sucrose + 8 g·L-1 agar; the optimum medium for rooting was MS +0.5 mg·L-1 NAA + 30 g·L-1 sucrose + 8 g·L-1 agar. The tissue culture seedlings grew well and the living ratio reached 93% after planted for 20 d. The establishment of rapid propagation technique system of red-flowered strawberry provides scientific and technological support for large-scale production of high quality red-flowered strawberry seedlings.关键词
红花草莓/组织培养/快繁技术Key words
red-flowered strawberry/tissue culture/rapid propagation分类
农业科技引用本文复制引用
金美芳,曹智,蔡俊杰,林茂兹..红花草莓的组织培养与快繁技术研究[J].广西植物,2017,37(11):1395-1405,11.基金项目
国家自然科学基金(31370589) （31370589）
福建省教育厅A类项目基金(JA14340)[Supported by National Natural Science Foundation of China(31370589) （JA14340）
Educational Commission of Fujian Province(JA14340)]. （JA14340）
