河北医学2017，Vol.23Issue(11)：1768-1772,5.DOI:10.3969/j.issn.1006-6233.2017.11.003
宫颈癌及癌前病变中的HPV-16整合人宿主基因组的发生情况及其在宫颈癌筛查中的应用
Integrated State of HPV-16 in Precancerous Tissues and Cervical Cancer and Application in Cervical Cancer Screening
摘要
Abstract
Objective:To investigate the prevalence of physical state of HVP-16 DNA in cervical and cervical precancerous carcinoma. Method:Multiplex PCR was adopted to detect the physical state of HPV in samples from 252 patients with cervical carcinoma, including 48 samples of cervical cancer, 204 cervical in-traepithelial(CIN)(125 CIN I,46 CIN Ⅱand 33 CINⅢ)and 20 normal samples from the subjects with hys-teromyoma undergoing hysterectomy ,respectively. Result:Not only patients with cervical cancer but also pa-tients with precancerous were found to have integrated infection with HPV-16 and those with cervical cancer have the highest integration rate. With the elevating of the grades of CIN, the integrated infection rates of HVP-16 was significantly elevated, the difference was statistically significant (χ2=49.7359,12.6033, P<0.01). Conclusion:Among the patients with HPV-16 infection,the integrated state of HVP-16 is positively correla-ted with the severity of cervical lesions. Combined HVP typing test and detection of integrated viral contribute to predicting the prognosis of patients with cervical precancerous lesions and increasing the accuracy of screen-ing cervical cancer on the basis of HPV DNA detection.关键词
宫颈癌/癌前病变/人类乳头瘤病毒/整合Key words
Cervical cancer/Precancerous tissues/Human papillomavirus/Integration引用本文复制引用
王霞..宫颈癌及癌前病变中的HPV-16整合人宿主基因组的发生情况及其在宫颈癌筛查中的应用[J].河北医学,2017,23(11):1768-1772,5.基金项目
陕西省科技攻关项目,(编号:201205319) （编号:201205319）
