华南农业大学学报2017，Vol.38Issue(6)：1-8,8.DOI:10.7671/j.issn.1001-411X.2017.06.001
饮用水中添加脂多糖对小鼠能量代谢及行为的影响
Effects of adding lipopolysaccharide to drinking water on energy metabolism and behavior of mice
摘要
Abstract
[Objective]To investigate the effects of long-term drinking water supplemented with lipopolysaccharide (LPS) on feeding, energy metabolism and behavior of mice.[Method]Totally 48 five-week-old C57/BL mice were randomly divided into eight groups. Different concentrations of LPS were injected intraperitoneally to test food intake. Meanwhile, 24 five-week-old C57/BL mice were randomly divided into three groups including control group without LPS, O128:B12 group (adding 5 μg·mL–1 Escherichia coil O128:B12 LPS to drinking water) and O55:B5 group (adding 5 μg·mL–1 E. coil O55:B5 LPS to drinking water). The experiment period was 12 weeks. Open field test and elevated plus-maze test were used to analyze the movement and anxiety behavior of mice. At the end of experiment, the body composition was tested and samples of fat tissue and brain nucleus were collected for gene expression analysis.[Result]Intraperitoneal injection of high dose LPS inhibited food intake of mice. Adding 5 μg·mL–1 LPS from different bacteria to drinking water significantly increased food intake of mice (P<0.05), and upregulated AgRP mRNA expression in the hypothalamic arcuate nucleus, while POMC mRNA expression was not affected. Drinking LPS significantly reduced feed efficiency, decreased fat deposition, and promoted the expression of UCP-1 and PGC-1ɑ in brown fat. Drinking LPS did not influence mouse anxiety behavior, but significantly promoted the locomotor activity of mice. Drinking LPS also significantly upregulated mRNA expression of c-fos and DRD2 in hippocampus.[Conclusion]Adding E. coli LPS to drinking water could improve mouse appetite, promote adipose metabolism of mice and increase locomotor activity of mice, which might be related to the increased activity of dopamine neurons in the hippocampus.关键词
脂多糖/采食/小鼠/自发运动/海马体基因表达Key words
lipopolysaccharide/food intake/mouse/locomotor activity/hippocampus gene expression分类
生物科学引用本文复制引用
袁业现,余璐璐,廖正睿,朱灿俊,蔡兴才,朱晓彤,江青艳,束刚,高萍..饮用水中添加脂多糖对小鼠能量代谢及行为的影响[J].华南农业大学学报,2017,38(6):1-8,8.基金项目
国家自然科学基金(31572480) （31572480）
国家重点研发计划(2016YFD0501205) （2016YFD0501205）
