华南农业大学学报2017，Vol.38Issue(6)：14-19,6.DOI:10.7671/j.issn.1001-411X.2017.06.003
虾青素对小鼠肝脏原代细胞氧化应激的影响
Effects of astaxanthin on oxidative stress response in primary mouse hepatocytes
摘要
Abstract
[Objective]To investigate the effect of astaxanthin on H2O2-induced oxidative stress response in primary mouse hepatocytes.[Method]Primary mouse hepatocytes were isolated by an in situ two-step perfusion technique. The model of oxidative stress response in primary mouse hepatocytes was established using H2O2 treatment and malondialdehyde(MDA) as an indicating index. The content of reative oxygen species (ROS) and change in apoptosis rate were measured by flow cytometry. The contents of MDA and glutathione(GSH), and the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-px) were measured by biochemical methods. The relative mRNA expression levels of antioxidant enzymes including SOD and GSH-px were measured by fluorescence quantitative PCR. The relative expression of Nrf2 protein was measured by Western-blot.[Result]The model of oxidative stress response performed the best with the primary mouse hepatocytes pre-protected by 5 μg·mL–1 astaxanthin for 3 h before being exposed to 10μmol·L–1H2O2 for 3 h. Astaxanthin decreased the apoptosis rate and the contents of ROS, MDA and GSH in primary mouse hepatocytes induced by H2O2. Astaxanthin increased the activities of SOD and GSH-px as well as the relative mRNA expression levels of SOD and GSH-px, and inhibited the nuclear transfer of Nrf2 protein.[Conclusion]Astaxanthin has protective effects on H2O2-induced primary mouse hepatocytes with oxidative stress.关键词
小鼠肝脏原代细胞/虾青素/抗氧化酶/Nrf2蛋白/H2O2Key words
primary mouse hepatocyte/astaxanthin/antioxidant enzyme/Nrf2 protein/H2O2分类
农业科技引用本文复制引用
张珊珊,吴旻,张晓音,兰海楠,郑鑫..虾青素对小鼠肝脏原代细胞氧化应激的影响[J].华南农业大学学报,2017,38(6):14-19,6.基金项目
国家自然科学基金(31672511) （31672511）
