华南农业大学学报2017，Vol.38Issue(6)：20-26,7.DOI:10.7671/j.issn.1001-411X.2017.06.004
四带无须鲃透明品系的海分枝杆菌感染模型
A Mycobacterium marinum infection model for transparent tiger barb Puntius tetrazona
摘要
Abstract
[Objective]To study the characteristics of transparent tiger barb Puntius tetrazona infected with Mycobacterium marinum and develop an infection model.[Method]P. tetrazona were infected with M. marinum via intraperitoneal injection, intramuscular injection and cohabitation experiment. The fish characteristics after infection were observed. Distribution of bacteria and pathological changes in the fish were evaluated by wet-mount preparations and tissue slices.[Result]Both injection methods resulted in pathologic damages around the infection sites, and gradually led to fish death. The disease progression was slow for the fish in the cohabitation experiment, and the fish appeared thin and pale. The pathogenic bacteria were isolated from fish with different infection treatments. Granulomas, the classic symptom for infection, were observed in major organs. M. marinum could be specifically stained by Ziehl-Neelsen stain. A number of melano-macrophage centers developed in different organs, and the organs were associated with different degrees of pathologic lesion.[Conclusion]The transparent line of tiger barb is a susceptible host of M. marinum, and the symptoms can be conveniently observed with typical pathological changes. P. tetrazona is an excellent model for further infection research.关键词
四带无须鲃/透明品系/海分枝杆菌/感染模型Key words
Puntius tetrazona/transparent line/Mycobacterium marinum/infection model分类
生物科学引用本文复制引用
王芳,刘春,姜兰,冯永永,李凯彬..四带无须鲃透明品系的海分枝杆菌感染模型[J].华南农业大学学报,2017,38(6):20-26,7.基金项目
国家科技支撑计划(2015BAI09B05) （2015BAI09B05）
中国水产科学研究院基本科研业务费(2014A06XK08) （2014A06XK08）
