华南农业大学学报2017，Vol.38Issue(6)：64-71,8.DOI:10.7671/j.issn.1001-411X.2017.06.010
拟南芥细胞分裂素受体AHK3亚细胞 定位信号的研究
Analysis of subcellular localization signals of Arabidopsis cytokinin receptor protein AHK3
摘要
Abstract
[Objective]To study the subcellular localization signals of cytokinin receptors and related mechanisms.[Method]A series of expression vectors for subcellular localization of Arabidopsis histidine protein kinase 3 (AHK3), one of cytokinin receptor proteins in Arabidopsis, were constructed and transformed into Arabidopsis protoplast cells for transient expression. Laser confocal microscope was used to observe the subcellular localization of AHK3 protein.[Result]AHK3 is localized in endoplasmic reticulum(ER). Both N and C terminals contain ER localization sequences.[Conclusion]AHK3 carry out its functions in ER, such as perception of cytokinin, downstream signal transduction and interactions with other receptor proteins, and AHK3 contains multiple ER localization signals.关键词
拟南芥/细胞分裂素/AHK3/亚细胞定位/内质网Key words
Arabidopsis thaliana/cytokinin/AHK3/subcellular localization/endoplasmic reticulum分类
农业科技引用本文复制引用
吴委,王作伟,粟永英,甘爽,杜世平,蔡易,郭晋雅..拟南芥细胞分裂素受体AHK3亚细胞 定位信号的研究[J].华南农业大学学报,2017,38(6):64-71,8.基金项目
四川省教育厅重点项目(15ZA0001) （15ZA0001）
