华南农业大学学报2017，Vol.38Issue(6)：72-78,7.DOI:10.7671/j.issn.1001-411X.2017.06.011
表面活性剂对加利利链霉菌AF1发酵的影响
Effects of surfactants on fermentation of Streptomyces galilaeus AF1
摘要
Abstract
[Objective]To study the effects of surfactants on fermentation of Streptomyces galilaeus AF1 strain.[Method]The single factor experiments were performed to optimize the surfactant type and the initial concentration and determine the best adding time. The Box-Behnken test design and response surface analysis were used to determine the most appropriate concentrations of surfactants. Flow cytometer instrument was used to measure the cell fluorescence intensity and investigate the effects of surfactant on cell membranes of AF1.[Result]The optimum concentrations of added surfactants were 8.95 g·L–1 Tween-80, 2.09 g·L–1 Triton X-100 and 3.89 g·L–1 sorbitol. The best adding time was five days after inoculation. The membrane permeability test results showed that the cell fluorescence intensity of AF1 of the test group was significantly higher than that of the control group.[Conclusion]The response surface model explained 94.89% of the variation in the dependent variables and therefore had practical significance. Membrane permeability of the AF1 strain significantly increased after adding the optimal surfactants complex, which led to increased efflux of intracellular active substance and biosythesis of antibacterial substance.关键词
加利利链霉菌/AF1菌株/表面活性剂/发酵/响应面/细胞膜通透性Key words
Streptomyces galilaeus/AF1 strain/surfactant/fermentation/response surface/cell membrane permeability分类
农业科技引用本文复制引用
贺玉广,周思,胡晓云,廖美德..表面活性剂对加利利链霉菌AF1发酵的影响[J].华南农业大学学报,2017,38(6):72-78,7.基金项目
农村科技领域项目(2014A020208088) （2014A020208088）
