海洋科学2017，Vol.41Issue(7)：1-8,8.DOI:10.11759/hykx20170225001
凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析
Molecular cloning of hexokinase from Parachlorella kessleri and its expression analysis under different trophic modes
摘要
Abstract
cDNA(GenBank ID:AHF54566)encoding hexokinase(termed CkeHK)from the green alga Parachlorella kessleri was cloned and sequenced to understand the response to the presence and uptake of glucose at the molecular level.The transcriptional expression patterns of CkeHK were observed under phototrophic,heterotrophic,and mixotro-phic culture conditions.The results indicated that the CkeHk cDNA was 1,844 base pairs(bp)long,with an open reading frame (ORF) of 1 389 bp encoding 462 amino acids, with a calculated molecular mass of 49.73 kDa and an estimated isoelectric point of 6.98.Under the phototrophic condition as control,the transcriptional profiles of CkeHk showed that it could be upregulated under the heterotrophic and mixotrophic culture conditions.The mRNA level of CkeHK under the mixotrophic culture condition could be upregulated more than that under the heterotrophic culture condition. These re-sults suggest that light may play an important role in regulating the role of hexokinase in metabolizing exogenous glu-cose. These findings provide us valuable information for exploring the mechanism of metabolizing glucose in P. kessleri.关键词
凯氏拟小球藻(Parachlorellakessleri)/己糖激酶/基因克隆/表达分析Key words
Parachlorella kessleri/hexokinase/molecular clone/transcript expression分类
生物科学引用本文复制引用
陈军,崔红利,赵佳琳,秦松..凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析[J].海洋科学,2017,41(7):1-8,8.基金项目
国家重点研发计划项目(2016YFF0202304) （2016YFF0202304）
国家自然科学基金面上项目(41376139) （41376139）
烟台市科技计划项目(2016JHZB007) National Key Research and Development Program-China, No.2016YFF0202304 （2016JHZB007）
National Natural Science Foundation of China, No.41376139 （）
Science and Technology Program of Yantai City, No.2016JHZB007 （）
