海洋科学2017，Vol.41Issue(7)：9-15,7.DOI:10.11759/hykx20170112002
烟台市区河流入海口微生物群落的分析
Analysis of microorganism community in river estuaries of Yantai
摘要
Abstract
A total of 12 water samples were collected from four river estuaries of Yantai city (Xin, an River, Guangdang River, Yuniao River, and Jiahe River, respectively) in April 2015. The structure of the bacterial com-munity and the dominant groups were examined by PCR-Denatured Gradient Gel Electrophoresis based on 16S rRNA PCR amplification(PCR-DGGE). Results of the analysis demonstrated the presence of high diversities and enrichment in the four river estuaries. Each of the 12 samples showed more than 30 bands, and the Shannon bacte-rial diversity index varied from 3.3 to 3.61. The Shannon bacterial diversity index was higher in Xinan River and Guangdang River, lower in Yuniao River, and the lowest in Jiahe River. The cluster analysis, by UPGMA analysis, showed that the closer the geographical position was, the higher was the similarity of the bacteria. A total of 14 major DGGE bands were cloned and sequenced. Phylogenetic analysis of the 14 samples showed that Proteobacteria and Bacteroidetes were dominant in the four river estuaries. These results were highly consistent with those of a previous study on the diversity of bacterial communities in the coastal areas of Shandong province. This study could be useful for protecting the environment in the river estuaries of Yantai.关键词
烟台/河流入海口/PCR-DGGE/微生物多样性分析Key words
Yantai/river estuaries/PCR-DGGE/microorganism diversity analysis分类
天文与地球科学引用本文复制引用
蒲洋,李文军,王凯,赵彦宏,秦松..烟台市区河流入海口微生物群落的分析[J].海洋科学,2017,41(7):9-15,7.基金项目
鲁东大学科研启动基金(ly2014041) （ly2014041）
山西省煤基重点科技攻关项目(FT2014-01) Ludong University Research Initiation Funds, No.ly2014041 （FT2014-01）
Key Project of Coal-based Science and Technology in Shanxi Province, No. FT2014-01 （）
