海洋科学2017，Vol.41Issue(7)：37-43,7.DOI:10.11759//hykx20170119006
大菱鲆精子运动特征与精液pH的相关性研究
Analysis of turbot (Scophthalmus maximus) sperm motion characteristics and relationship with pH
摘要
Abstract
This study investigated the relationship between semen quality and pH of turbot(Scophthalmus maximus) and the impact of glycerin on turbot sperm activation using turbot sperm from different hatcheries (A, B, C, and D) through the computer-assisted sperm analysis (CASA) system. The results showed that sperm motility rates were different in the four hatcheries, A2 > A1 > D > C > B. The predicted sperm longevity times were 11.7, 7.1, 6.8, 4.6, and 3.3 min, respectively. The motility rates of turbot sperm significantly positively correlated with VCL, VSL, VAP, ALH, and BCF (P <0.05), but no significant correlation was observed with LIN, WOB, and STR. We found that the pH of turbot sperm significantly positively correlated with motility, VCL, VSL, VAP, ALH, and BCF(P < 0.05), whereas no significant correlation was found with LIN, WOB, and STR. In addition, high-quality sperm exhibited a weak alkalinity with a pH of 8.0. We also found that an appropriate concentration of glycerol (100 mmol/L) in sea water was useful for main-taining the longevity.关键词
大菱鲆(Scophthalmusmaximus)/精子/运动特征/pHKey words
Scophthalmus maximus/sperm/motion characteristics/pH分类
生物科学引用本文复制引用
安皓,徐世宏,丰程程,王彦丰,刘清华,李军..大菱鲆精子运动特征与精液pH的相关性研究[J].海洋科学,2017,41(7):37-43,7.基金项目
国家自然科学基金资助项目(31472264, 31572602) （31472264, 31572602）
鳌山科技创新计划项目 (2015ASKJ02, 2015ASKJ02-03-03) （2015ASKJ02, 2015ASKJ02-03-03）
现代农业产业技术体系项目(CARS-47) National Natural Science Foundation of China, No.31472264, 31572602 （CARS-47）
the Scientific and Technological Innovation Project , No.2015ASKJ02, 2015ASKJ02-03-03 （）
Modern Agro-industry Technology Research System, No.CARS-47 （）
