激光生物学报2017，Vol.26Issue(5)：438-442,5.DOI:10.3969/j.issn.1007-7146.2017.05.009
钙结合蛋白CIB1在293 T细胞中的表达及亚细胞定位研究
A Study on the Expression and Subcellular Localization of Calcium and Integrin Binding Protein 1 in 293 T Cells
摘要
Abstract
Alignment of CIB1 protein sequence among human,rat and mouse,and study on the expression and subcul-luar localization of CIB1 in 293T cells. Western blot analysis show that CIB1 protein expression is increased significantly in transfected 293T cells, but undetectable in untransfected 293T cells. To further monitor the expression and subcellu-lar localization of CIB1 in 293T cells,transfections CIB1 exogenous plasmid transfection with varying time were detected by immunofluorescence analysis in laser confocal microscope. The results show that with transfection of CIB1 exogenous plasmid into 293T cells, more and more CIB1 were expressed,and there are obvious translocation of CIB1 from nuclear to cytoplasm. These results will highlight the understanding of CIB1 subculluar localization changes its potential roles. Key words:calcium binding protein; CIB1;293T cells; laser scanning confocal microscope关键词
钙结合蛋白/CIB1/293Tcells/激光共聚焦显微镜Key words
calcium binding protein/CIB1/293T cells/laser scanning confocal microscope分类
生物科学引用本文复制引用
汪献旺,徐涵一,朱定良..钙结合蛋白CIB1在293 T细胞中的表达及亚细胞定位研究[J].激光生物学报,2017,26(5):438-442,5.基金项目
国家自然科学基金项目(31700736) （31700736）
湖北省自然科学基金项目(2016CFB180) （2016CFB180）
湖北省教育厅科学技术研究项目(Q20171306) （Q20171306）
湖北省卫计委项目(WJ2016Y07) （WJ2016Y07）
荆州市科技发展计划项目(JZKJ15063) （JZKJ15063）
长江青年科技创新团队基金项目(2016CQT04) （2016CQT04）
长江大学大学生创新创业训练计划项目(2016133) （2016133）
