激光生物学报2017，Vol.26Issue(5)：458-465,457,9.DOI:10.3969/j.issn.1007-7146.2017.05.012
斑马鱼心脏发育候选基因Titin-Cap的CRISPR/Cas9打靶有效性分析
Targeting Effectiveness Analysis of Heart Developmental Candidate Gene Titin-Cap with Gene Knockout Method CRISPR/Cas9
摘要
Abstract
CRISPR/Cas9 technology,developed rapidly in recent years with a high targeting efficiency,is considered a revolutionary field of genetic technology. Titin-Cap gene is a candidate gene for zebrafish cardiac development,and there is no knockout strain of zebra fish Titin-Cap gene in the world so far. In order to study the mechanism of the Titin-Cap gene in heart development, here the CRISPR/Cas9 gene targeting technique was used to establish the Titin-Cap gene knockout strain in zebrafish. The sequencing results showed that the embryos injected with CRISPR/Cas9 gRNAs had bi-modals, indicating that there was a base deletion or insertion near the target site. The sequencing results demonstrated that the gRNA was effective. In the screened F0 mutants,the sequencing results also showed positive results. All these results indicated that the Titan-Cap gene of the zebrafish was successfully knocked out with the CRISPR/Cas9 gene tar-geting technique, and the zebra fish of the Titin-Cap gene knockout was obtained.关键词
心脏发育/斑马鱼/CRISPR/Cas9/Titin-Cap基因Key words
heart development/zebrafish/CRISPR/Cas9/Titin-Cap gene分类
生物科学引用本文复制引用
袁月亲,罗世锋,颜尔聪,尹丽阳,漆轲婧,彭亚欣,万永奇,吴秀山,李永青..斑马鱼心脏发育候选基因Titin-Cap的CRISPR/Cas9打靶有效性分析[J].激光生物学报,2017,26(5):458-465,457,9.基金项目
国家自然科学基金面上项目(编号81470377) （编号81470377）
湖南省生物发育工程及新产品研发协同创新中心(2013-448-6) （2013-448-6）
