扬州大学学报（农业与生命科学版）2017，Vol.38Issue(3)：27-31,5.DOI:10.16872/j.cnki.1671-4652.2017.03.006
齐墩果酸抑制脂多糖诱导RAW264.7细胞炎症反应
Inhibition of oleanolic acid on lipopolysaccharide induced RAW264.7 cell inflammatory response
摘要
Abstract
CCK-8 assay was used to detect the influence of oleanolic acid (OA) on macrophage proliferation;Griess assay was used to determine the NO release in LPS induced macrophage with or without OA treatment;Western-blot method was used to detect the iNOS protein expression and MAPK pathway p38,ERK and JNK protein expression and phosphorylation status.To study the anti-inflammatory effect of OA on lipopolysaccharide (LPS) induced RAW264.7 macrophage and its mechanism.Results:only OA (100.0 μmol · L-1) inhibited macrophage proliferation.(P＜0.05);12.5-100.0 μmol · L-1 of OA significantly inhibited the secretion of NO in LPS stimulated macrophages (P＜0.01),and strongly inhibited the expression of iNOS protein;LPS activated the phosphorylation of ERK pathway,and inhibited the phosphorylation status of JNK and p38,but OA decreased the phosphorylation of ERK,and promoted the phosphorylation status of JNK and p38 protein.This study demonstrated that OA showed strong anti-inflammatory effect by inhibition of the expression of iNOS and regulation of protein phosphorylation of MAPK pathway in dual way.关键词
齐墩果酸/脂多糖/巨噬细胞RAW264.7/一氧化氮/MAPK通路Key words
Oleanolic acid/lipopolysaccharide/macrophages RAW264.7/nitric oxide/MAPK pathway分类
医药卫生引用本文复制引用
吴青青,王娟,崔树娜,陈姗姗,雷雨,李士华,钱静..齐墩果酸抑制脂多糖诱导RAW264.7细胞炎症反应[J].扬州大学学报（农业与生命科学版）,2017,38(3):27-31,5.基金项目
国家自然科学基金资助项目(81703969) （81703969）
江苏省自然科学基金资助项目(BK20160480) （BK20160480）
