扬州大学学报（农业与生命科学版）2017，Vol.38Issue(3)：32-38,7.DOI:10.16872/j.cnki.1671-4652.2017.03.007
丹参中转录因子基因SmMYB-like 1的克隆和表达分析
Cloning and expression analysis of a transcription factor gene SmMYB-like 1 in Salvia miltiorrhiza
摘要
Abstract
We obtained a full-length cDNA sequence of R2R3 MYB transcription factors with the homology-based cloning and RACE methods.This gene was named SmMYB-like 1 (GenBank accession No.:JX997333),it contained a 960 bp open reading frame (ORF) encoding 319 amino acids.Sequence analysis predicted that molecular weight of SmMYB-like 1 was 35.84 Ku,and the academic isoelectric point of this protein was 6.24.The multiple sequence alignment and phylogenetic analysis were applied,and the second structure as well as 3-D structure of SmMYB-like 1 was predicted.QRT-PCR was performed to determine expression levels of SmMYB-like 1 in different tissues (root,stem,leaf and flower) of S.miltiorrhiza.Results showed that SmMYB-like 1 expressed in all the tissues tested,with the lowest expression in root and much higher expression in leaf and flower.The recombinant constructs of the SmMYB-like 1-GFP fusion gene was introduced into onion epidermal cells by particle to determine the subcellular localization of SmMYB-like 1 protein,the results showed that it distributed both in nucleus and cytomembrane.关键词
丹参/R2R3 MYB/基因克隆/亚细胞定位/表达分析Key words
Salvia miltiorrhiza/R2R3 MYB/gene cloning/subcellular localization/expression analysis分类
农业科技引用本文复制引用
张顺仓,夏鹏国,王幼平,梁宗锁..丹参中转录因子基因SmMYB-like 1的克隆和表达分析[J].扬州大学学报（农业与生命科学版）,2017,38(3):32-38,7.基金项目
国家自然科学基金资助项目(31700257、81773835) （31700257、81773835）
江苏省高校自然科学基金面上项目(16KJB180032) （16KJB180032）
中国博士后科学基金面上项目(2015M571826) （2015M571826）
