扬州大学学报（农业与生命科学版）2017，Vol.38Issue(3)：50-56,7.DOI:10.16872/j.cnki.1671-4652.2017.03.010
湖羊IGF-Ⅰ和MyoG基因表达的发育性变化及其与屠宰性状的关联分析
Developmental changes of gene expression of IGF-Ⅰ and MyoG genes and their association analysis with carcass traits in Hu sheep
摘要
Abstract
In this study,Hu sheep was researched object and methods of RT-PCR was applied to analyze the expression of IGF-Ⅰ and MyoG in Hu sheep longissimus dorsi at different growth stages and their association analysis with meat quality traits,and the relationship of the expression level between IGF-Ⅰ and MyoG.The objective was is to reveal the genetic background of muscle growth in Hu sheep by using candidate gene method and provide genetic information for the selection of muscle growth in Hu sheep.The results were as follows:① With increasing age after birth,the IGF-Ⅰ expression trend of ewes was increasing from 2-day to 6-month which had the top expression;the IGF-Ⅰ expression trend of rams was that 2-day firstly increased to a peak at one-month,1-month to 3-month had a decreasing process and reached the same level which was close to the newborn,and then inceased with the increasing age,6-month reached the top.With increasing age after birth,the MyoG expression trend of ewes was up-down-up-down-up,and 6-month reached the top;the MyoG expression trend of rams was that 2-day firstly increased to a peak at one-month,1-month to 4-month was a process of decreasing,4-month gradually increased to 6-month which reached top expression.Most growth phases had the significant difference or extreme significant difference in IGF-Ⅰ and MyoG and the expressions of IGF-Ⅰ and MyoG between most rams and ewes that at the same growth phase had the significant difference or extreme significant difference.② The expressions of IGF-Ⅰ and MyoG had an extreme significant positive correlation,the expressions of IGF-Ⅰ had an extreme significant positive correlation with live weight and a significant positive correlation with carcass weight and had no significant correlation with net meat weight;the expression of MyoG had no significant correlation with live weight,carcass weight and net meat weight.The results showed that IGF Ⅰ and MyoG had an extreme significant positive effect on Hu sheep early muscles growth.This effect was affected by age and gender,and there may be a gender specificity.关键词
湖羊/IGF-Ⅰ基因/MyoG基因/基因表达/屠宰性状Key words
Hu sheep/IGF-Ⅰ gene/MyoG gene/gene expression/carcass trait分类
农业科技引用本文复制引用
王利宏,高雯,鲍建军,于嘉瑞,孙伟..湖羊IGF-Ⅰ和MyoG基因表达的发育性变化及其与屠宰性状的关联分析[J].扬州大学学报（农业与生命科学版）,2017,38(3):50-56,7.基金项目
国家星火计划重点项目(2012GA690003) （2012GA690003）
江苏省农业科技支撑计划项目(BE2012331) （BE2012331）
江苏省工程技术研究中心科研项目(BM2012308) （BM2012308）
科技部家养动物平台项目(2015-10) （2015-10）
江苏省高校优势学科建设工程项目(2014-10) （2014-10）
江苏省苏北科技计划项目(BN2014003、BN2015013、BN2015014) （BN2014003、BN2015013、BN2015014）
江苏省农业科技自主创新项目[CX(14)2073] （14）
江苏省研究生科研创新计划项目(KYLX16-1402) （KYLX16-1402）
