扬州大学学报（农业与生命科学版）2017，Vol.38Issue(3)：57-61,67,6.DOI:10.16872/j.cnki.1671-4652.2017.03.011
绵羊mtDNA COⅠ基因作为DNA条形码鉴定品种及系统进化可行性分析
The feasibility analysis of ovine mtDNA CO Ⅰ gene as DNA barcoding in breed identification and phylogenetic
摘要
Abstract
The mtDNA CO Ⅰ gene,of which Shandong Small-tailed Han sheep,Wadi sheep,Sunite sheep,Hebei Small-tailed Han sheep and Hu sheep,were obtained through mitochondrial genome sequencing.The population genetics and genetic diversity were analyzed by CO Ⅰ gene segment (58-705 bp),as well as the feasibility of mtDNA CO Ⅰ gene as barcoding gene was explored in ovine breed identification and phylogenetic in this experiment.The result indicated that the comparability of different sheep population was 99.1 ％-100.0％.From the haplotypes and distributions of populations,there were independent haplotypes which contained one individual in seven samples of Hebei Small-tailed Han sheep,however,the flock specific haplotype was no found.The above result demonstrated that CO Ⅰ gene (58-705 bp) was not suitable for population identification.The phylogenetic analysis showed that the Hebei Small-tailed Han sheep and Wadi sheep were clustered first,and then they clustered with Hu sheep.Further,they clustered with Shandong Small-tailed Han sheep,Tan sheep and Sunite sheep.Ultimately,Altay sheep clustered with the above populations.The results were consistent with history migration and population evolution.The COⅠ gene segment (58-705 bp) could be used to phylogenetic analysis.However,it was not suitable for DNA barcoding which used to identify different sheep populations.This conclusion was further confirmed through more quantities and populations.关键词
绵羊/COⅠ基因/DNA条形码/品种鉴定/系统进化Key words
local breed/COⅠ gene/DNA barcodin/identify/breed identification/phylogenetic分类
农业科技引用本文复制引用
梁瑞圆,陈晓勇,孙洪新,沈志强,刘军峰,王珏,孙少华,敦伟涛..绵羊mtDNA COⅠ基因作为DNA条形码鉴定品种及系统进化可行性分析[J].扬州大学学报（农业与生命科学版）,2017,38(3):57-61,67,6.基金项目
河北省科技支撑计划项目(15226308D) （15226308D）
