扬州大学学报（农业与生命科学版）2017，Vol.38Issue(3)：116-120,5.DOI:10.16872/j.cnki.1671-4652.2017.03.020
花生过敏原蛋白Ara h2的分离纯化研究
Isolation and purification of peanut allergen protein Ara h2
摘要
Abstract
Food allergy is a prominent food safety problems,and peanut allergens were classified into the first of the eight allergens because of its high allergenicity.In peanut allergen,Ara h2 is one of the major allergens.In this experiment,Ara h2 as the research object,based on the defatted peanut,setting different extracts,pH,material liquid ratio,extraction time of the protein extraction conditions,the target protein was purified with dialysis,and twelve sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to identify the optimization results,and the optimum extraction process was determined by the orthogonal test.The results showed that the optimum extraction parameters were:extraction time 90 min,pH 7.4,liquid ratio 1:8,Tris-HCl buffer.Under this method,the content of target protein can reach 1.58 mg · mL-1,and the yield can reach 47.8％.关键词
花生过敏原蛋白Ara h2/条件优化/正交试验/纯化Key words
peanut allergen Ara h2/condition optimization/purification/orthogonal test分类
轻工纺织引用本文复制引用
蒋栋磊,葛攀玮,朱丽,俞程凯,葛庆丰,方维明..花生过敏原蛋白Ara h2的分离纯化研究[J].扬州大学学报（农业与生命科学版）,2017,38(3):116-120,5.基金项目
国家自然科学基金面上项目(31271945、31371792) （31271945、31371792）
国家自然科学基金青年科学基金项目(31601535) （31601535）
江苏省自然科学基金青年科学基金项目(BK20160459) （BK20160459）
江苏省高校自然科学资金面上项目(16KJB550008) （16KJB550008）
扬州市社会发展前瞻性研究项目(YZ2014177) （YZ2014177）
国家级大学生创新训练项目(201711117042Z) （201711117042Z）
扬州大学研究生实践创新计划项目(SJCX17_0631) （SJCX17_0631）
