扬州大学学报（农业与生命科学版）2017，Vol.38Issue(3)：121-126,6.DOI:10.16872/j.cnki.1671-4652.2017.03.021
盐酸克伦特罗喷射式流动注射电化学发光检测方法的研究
Study of the flow injection chemiluminescence detection method on clenbuterol
摘要
Abstract
A new flow injection electrochemiluminescence (ECL) analytical method of clenbuterol was proposed based on its sensitizing effect on the ECL of Ru(bpy)32+.The experiments investigated the effect of flow velocity and medium pH on the Ru(bpy)32+ ECL,and the effect of Ru(bpy)32+ concentration on the detection results of clenbuterol.Under optimum conditions,the relationship between the increased values of ECL intensity after adding melamine and its concentration was established.It was found that when flow velocity was 30 μL · s-1,medium pH was 9.0,the maximum ECL intensity of Ru(bpy)32+ was obtained.Under these conditions,the Ru(bpy)32+ concentration of 1.0 × 10-4 mol · L-1 was selected for the melamine detection.The increased values of ECL intensity after adding melamine was linearly correlated with the concentration logarithm of clenbuterol in the range of 1 × 10-10-1 ×(10-6 g · mL-1,with a detection limit of 1×10-10 g· mL-1.关键词
盐酸克伦特罗/Ru(bpy)32+/流动注射/电化学发光Key words
clenbuterol/Ru(bpy)32+/flow Injection/electrochemiluminescence分类
化学化工引用本文复制引用
张婷,谢晋雄,黄彬庚,詹爱军,王飞,李维,陈峰,吴绍精,陈勇..盐酸克伦特罗喷射式流动注射电化学发光检测方法的研究[J].扬州大学学报（农业与生命科学版）,2017,38(3):121-126,6.基金项目
“十二五”国家科技支撑计划项目(2012BAK17B08) （2012BAK17B08）
国家质检总局科研项目(2015IK245) （2015IK245）
深圳市出入境检验检疫局科研项目(SZ2014204) （SZ2014204）
