解放军医学院学报2017，Vol.38Issue(10)：922-926,5.DOI:10.3969/j.issn.2095-5227.2017.10.005
结直肠癌中hsa_circ_0006156RNA的低表达及其临床意义
Decreased expression of hsa_circ_0006156 in colorectal cancer and its significance
摘要
Abstract
Objective To investigate the expression of hsa circ 0006156 in colorectal cancer (CRC) and its significance.Methods The expression of hsa circ 0006156 in CRC tissues and adjacent normal tissues in 31 patients who underwent surgical treatment in our hospital from December 2014 to December 2015 was detected by real-time fluorescence-based quantitative PCR.The clinicopathological data were collected and analyzed to study its clinical significance.The interaction between hsa circ 0006156 and microRNAs was predicted and analyzed by bioinformatic software such as Blast and Bedtools.Results Compared with the normal tissues,hsa circ 0006156 was significantly down-regulated in cancer tissues with low expression in 24 patients (77.4％),while it was up-regulated in 7 patients (22.6％),and its expression was correlated with tumor size (P=0.042).ROC curve was constructed for differentiating CRC tissues from normal tissues.The AUC (area under curve) was up to 72.3％ (95％ CI:0.592-0.853),and the sensitivity and specificity of hsa circ 0006156 were 71％ and 77.4％,respectively.hsa circ 0006156 had binding sites of hsamiR-5011 (n=24),hsa-miR-4775 (n=22),hsa-miR-4445 (n=20),and hsa-miR-627 (n=12).Conclusion The expression of hsa_circ_0006156 is down-regulated in CRC and is a potential diagnostic biomarker for clinical diagnosis.hsa_circ_0006156 may act as miRNA sponge in post-transcriptional regulation.关键词
环形RNA/结直肠癌/hsa_circ_0006156 RNA/分子标记物Key words
circular RNA/coloretal carcinoma/hsa_circ_0006156/biomarker分类
医药卫生引用本文复制引用
黄亮,王绪宁,贾宝庆,刘洪一,郭少华,张加金,郑伟..结直肠癌中hsa_circ_0006156RNA的低表达及其临床意义[J].解放军医学院学报,2017,38(10):922-926,5.基金项目
国家高科技研究发展计划(2014AA020802)Supported by the National High Technology Research and Development Program of China(2014AA020802) （2014AA020802）
