检验医学与临床2017，Vol.14Issue(22)：3279-3281,3286,4.DOI:10.3969/j.issn.1672-9455.2017.22.001
fmnl2基因沉默抑制SW620体外侵袭能力的机制
Mechanism of fmnl2 gene silencing for inhibiting in vitro invasion ability of SW 620
摘要
Abstract
Objective To explore the mechanism of fmnl2 gene silencing for inhibiting the in vitro invasion ability of SW 620 . Methods SW620 cells with fmnl2 gene silencing were screened and established .specific shRNA plasmid vectors as well as con-trols .fmnl2 silenced cells were screened by G418 .The real time fluorescence quantitative RT-PCR and Western blot analysis were used to detect the interference efficiency ;the influence of fmnl on SW620 invasion ability was detected by the Boyden test .The ac-tive Rho pull-down test and Western blot were used to detect the Rho A ,Rho B ,Rho C activity and protein level change after fmnl2 gene silencing ;the 3 kinds of effector proteins Phospho MLC ,Phospho-LIMK Phospho Cofilin ,and F-actin ,intranuclear MAL pro-tein changes .The in vitro invasion ability of LPA on cells ,RhoA activity and protein level ,Phospho-MLC and MLE influence were detected before and after fmnl2 gene silencing .Results fmnl2 gene silencing significantly inhibited the SW620 cell invasion ability in vitro .After fmnl2 expression silencing ,the intracellular RhoA activity was decreased ,but the protein level had no obvious change;the protein levels of Phospho-Cofilin ,Phospho-LIMK ,Phospho-MLC ,F-actin and intranuclear MAL were decreased .After fmnl2 expression silencing ,LPA had no influence on the cell in vitro invasion ability ,PhoA-activity and Phospho-MLC . Conclusion fmnl2 silencing could influence the invasion and motor ability of tumor cells by inhibiting the RhoA/Rock signaling transduction pathway .关键词
大肠癌/fmnl2基因/DRFs家族/侵袭/Rho蛋白Key words
colorectal cancer/fmnl2 gene/DRFs family/invasion/Rho protein引用本文复制引用
朱曦龄,黎功,李黛..fmnl2基因沉默抑制SW620体外侵袭能力的机制[J].检验医学与临床,2017,14(22):3279-3281,3286,4.基金项目
国家自然科学基金资助项目(30400206 ). （30400206 ）
