检验医学与临床2017，Vol.14Issue(22)：3328-3329,3331,3.DOI:10.3969/j.issn.1672-9455.2017.22.018
二甲双胍对高糖环境下人肾小管上皮细胞的保护机制
Protective mechanism of metformin on human renal tubular epithelial cells under high glucose environment
摘要
Abstract
Objective To investigate the protective mechanism of metformin on human renal tubular epithelial cells (HK-2)under high glucose environment through observing its effect in this environment .Methods The high glucose culture mediums with differ-ent concentrations of metformin and pyrrolidine dithiocarbamate (PDTC)were adopted to culture HK-2 cells in vitro for 24 ,48 ,72 h ,and then the irregular chemokine(Fkn)protein expression level in HK-2 cells were detected by using MTT and Western blot .Re-sults Compared with the low glucose serum deprivation group ,the cell proliferation in the high glucose serum deprivation group was suppressed ;adding different levels of metformin could promote the cellular proliferation ,in which the effect of adding 30mmol/L of metformin for promoting cellular proliferation was most obvious ;adding PDTC in high glucose culture medium also could re-verse the effect of high glucose for inhibiting dellular proliferation .The Western blot results showed that Fkn protein expression in the high glucose serum deprivation group was significantly increased ,with the concentration increase of added metformin ,the Fkn protein level was decreased significantly ,in adding 30 mmol/L of metformin and culturing for 72 h ,the descend range was the most obvious ,moreover which showed a time dependence .Conclusion High glucose status can inhibit the proliferation of HK-2 cells ,re-duces the secretion of Fkn protein ,while 30 mmol/L and above of metformin can protect the high glucose induced HK-2 prolifera-tion by inhibiting Fkn protein secretion .关键词
人肾小管上皮细胞/二甲双胍/不规则趋化因子/糖尿病肾病Key words
HK-2/metformin/Fkn/diabetic nephropathy引用本文复制引用
乔青燕,金惠敏,顾燕红,顾惠芳,付顺昆,潘丽华..二甲双胍对高糖环境下人肾小管上皮细胞的保护机制[J].检验医学与临床,2017,14(22):3328-3329,3331,3.基金项目
上海市浦东新区医学领先人才项目(PWRl2012-05) （PWRl2012-05）
上海市浦东新区卫生系统重点学科建设项目(PWZx2014-11) （PWZx2014-11）
上海市医学重点专科建设项目(ZK2015A15). （ZK2015A15）
