临床口腔医学杂志2017，Vol.33Issue(11)：643-647,5.DOI:10.3969/j.issn.1003-1634.2017.11.002
PELP1在炎性牙周膜细胞中的表达及与细胞增殖和成骨分化的相关性研究
Expression of PELP1 in inflammatory periodontal ligament cells and its correlation with cell proliferation and ostegenic differentiation
摘要
Abstract
Objective:To investigate the relationship between PELP1 and bone differentiation in periodontal ligament cells. Methods:Human periodontal ligament cells( hPDLCs) were cultured and identified by immunocytochemistry. RT-PCR and Western blot were performed to detect PELP1 expression on proliferation and osteogenic differentiation. Results:The results showed that PELP1 was expressed in both H-hPDLFCs and Ⅰ-hPDLFCs. Compared with PELP1 expression in H-hPDLF-Cs,the PELP1 level of Ⅰ-hPDLFCs decreased with culture time. During osteogenic induction,the PELP1 expression in Ⅰ-hP-DLFCs was decreased with culture time,and similar with the expression of the osteogenic marker osteocalcin. Conclusion:PELP1 expressed in hPDLCs and played a role in PDLCs' proliferation and osteogenic differentiation.关键词
牙周膜细胞/PELP1/成骨分化/增殖Key words
Human periodontal ligament cells( hPDLCs)/PELP1/Osteogenic induction/Proliferation分类
生物科学引用本文复制引用
王婧,祝强,李学盛,庞恋苏,王燕一..PELP1在炎性牙周膜细胞中的表达及与细胞增殖和成骨分化的相关性研究[J].临床口腔医学杂志,2017,33(11):643-647,5.基金项目
全军医学科技青年培育项目(15QNP076) （15QNP076）
海南省自然基金项目(20168360) （20168360）
