临床口腔医学杂志2017，Vol.33Issue(11)：656-659,4.DOI:10.3969/j.issn.1003-1634.2017.11.006
枸杞多糖诱导自噬抑制人舌鳞状癌细胞增殖
The autophagy and apoptosis induced by Lycium barbarum Polysaccharide in human tongue squamous cancer cells
摘要
Abstract
Objective:To investigate the effect and mechanism of Lycium barbarum Polysaccharides( LBP) on human tongue squamous cancer CAL-27 cells via induce autophagy.Methods:CAL-27 cells were treated with different concentration of LBP. Proliferation was tested by CCK-8. The apoptosis and cell cycle were analyzed by flow eytometry. Expression of autophagy-related protein LC3 was determined by immunofluorescence. Results:LBP could inhibit the proliferation of CAL-27 cells,induce cell apoptosis and arrest the cell cycle in the G2 phase with a dose-effect relationship. The LC3-Ⅱ protein expression following LBP treatments increased significantly with a dose-effect relationship,which suggested that LBP can regulate the expression of LC3 to induce autophagy. Conclusion:LBP might induce CAL-27 cell autophagy and apoptosis to significantly inhibit cell proliferation.关键词
枸杞多糖/细胞自噬/人舌鳞状癌/CAL-27细胞Key words
Lycium barbarum Polysaccharides/Autophagy/Human tongue squamous cancer/CAL-27分类
生物科学引用本文复制引用
姜鸣,李卓能..枸杞多糖诱导自噬抑制人舌鳞状癌细胞增殖[J].临床口腔医学杂志,2017,33(11):656-659,4.基金项目
武汉市卫计委重点科研项目(WG15A01) （WG15A01）
