临床与实验病理学杂志2017，Vol.33Issue(7)：737-741,5.DOI:10.13315/j.cnki.cjcep.2017.07.007
长链非编码RNA-HOTAIR对子宫内膜癌转移侵袭能力的影响
Influence of long chain non-coding RNA (lncRNA)-HOTAIR on migration and invasion ability of endometrial cancer
摘要
Abstract
Purpose To investigate the influence of long chain non-coding RNA (lncRNA)-HOTAIR on endometrial cancer cell proliferation,invasion,metastasis and other biological behaviour.Methods 20 cases of endometrial carcinoma tissue specimen,20 cases of hyperplasia tissue sample and 10 cases of normal tissue specimen were collected.Difference expression of lncRNA-HOTAIR in normal endometrium,hyperplasia endometrium tissue and endometrial carcinoma tissue at all periods were detected with RT-PCR assay.HOTAIR-siRNA transfection into Ishikawa cells was utilized with Lipofectamine 2000.MTT experiment was used to detected the proliferation ability of cells in all groups.Transwell chamber experiment was used to test the migration and invasion ability of cells in all groups.Results The gene expression level of of lncRNA-HOTAIR in endometrial carcinoma group at all stages was prominently increased compared with normal endometrium group (P ＜ 0.05).The expression level of lncRNA-HOTAIR in simple hyperplasia endometrium group and atypical hyperplasia endometrial group was not significantly different (P ＞ 0.05).Cell proliferation,invasion ability and migration ability of HOTAIR-siRNA targeting suppression group were lower than the blank control group and the negative control group significantly (P ＜ 0.05).Conclusion lncRNA-HOTAIR may involved in occurrence and development of endometrial cancer,which may play an important role in the aggression and metastasis of endometrial cancer.关键词
子宫肿瘤/子宫内膜癌/lncRNA-HOTAIR/siRNA/迁移与侵袭Key words
endometrial neoplasm/endometrial cancer/lncRNA-HOTAIR/siRNA/migration and invasion分类
医药卫生引用本文复制引用
王明娟,周晓慧,刘超..长链非编码RNA-HOTAIR对子宫内膜癌转移侵袭能力的影响[J].临床与实验病理学杂志,2017,33(7):737-741,5.基金项目
河北省卫生计生委(ZD20140070) （ZD20140070）
