临床与实验病理学杂志2017，Vol.33Issue(7)：754-758,5.DOI:10.13315/j.cnki.cjcep.2017.07.011
肺腺癌中PD-1、PD-L1蛋白表达与K-RAS基因突变状态的相关性分析
Relevant research of PD-1, PD-L1 protein expression and K-RAS gene mutations in lung adenocarcinoma
摘要
Abstract
Purpose To investigate the association of the PD-1 and PD-L1 protein expression with K-RAS gene mutations in lung adenocarcinoma.Methods The protein expression of PD-1 and PD-L1 was detected by immunohistochemical EnVision two-step staining,the K-RAS mutation was examined by realtime fluorescent quantitative PCR.Results The positive rate of PD-1 and PD-L1 was higher in lung adenocarcinoma than benign lung disease (P ＜ 0.01).There was no relationship between PD-1 and PD-L1 protein expression with the gender,age,smoking condition,differentiation,lymph node metastasis and TNM stages (P ＞ 0.05).The K-RAS gene mutations were detectable in 8 patients (22.2％) among 36 lung adenocarcinoma,there was no association between K-RAS gene mutation with the gender,age,smoking condition,differentiation,lymph node metastasis and TNM stages (P ＞ 0.05).The correction analysis showed that there was no relationship between PD-1 and PD-L1 protein expression with K-RAS mutation (P ＞ 0.05).Conclusion The positive rate of PD-1 and PD-L1 is higher in lung adenocarcinoma than benign lung disease,but there is no relationship among PD-1 and PD-L1 protein expression with its clinal pathological characteristics and K-RAS mutation in lung adenocarcinoma.关键词
肺肿瘤/腺癌/PD-1/PD-L1/K-RAS基因突变Key words
lung neoplasm/adenocarcinoma/PD-1/PD-L1/K-RAS gene mutations分类
医药卫生引用本文复制引用
王珊,董丽儒,任会强,刘爱东,熊艳杰,宋旭东..肺腺癌中PD-1、PD-L1蛋白表达与K-RAS基因突变状态的相关性分析[J].临床与实验病理学杂志,2017,33(7):754-758,5.基金项目
2016年河北省政府资助临床医学优秀人才培养和基础课题研究(361036) （361036）
