临床与实验病理学杂志2017，Vol.33Issue(7)：759-762,4.DOI:10.13315/j.cnki.cjcep.2017.07.012
非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤MDM2蛋白表达和基因扩增检测及临床意义
Clinical significance of MDM2 protein expression and gene amplification in atypical lipomatous tumour/well-differentiated liposarcoma
潘丹玲 1陈小岩 1陈灵锋 1林瀛1
作者信息
- 1. 福建医科大学省立临床医学院福建省立医院病理科,福州350001
- 折叠
摘要
Abstract
Purpose This study was to investigate the clinical significance of MDM2 protein expression and gene amplification in atypical lipomatous tumour/well-differentiated liposarcoma (ALT/WDL).Methods MDM2 protein expression and gene amplification were detected by immunohistochemistry of EliVision two-step method and fluorescence in situ hybridization (FISH) in 58 cases of ALT/WDL.Results Immunohistochemical staining showed that the positive rate of MDM2 was 79.3％,MDM2 gene amplification rate by FISH assay was 96.5％.There was statistically significant differences between immunohistochemistry and FISH (P ＜ 0.05).Conclusion MDM2 protein expression and MDM2 gene amplification provide the basis for diagnosis of ALT/WDL.However,a positive rate of MDM2 by immunohistochemistry is nearly 80％,so we recommand to carry out immunohistochemical MDM2 protein detection in most conditions without FISH which can help in the diagnosis of most cases of ALT/WDL.关键词
非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤/MDM2/免疫组织化学/荧光原位杂交Key words
atypical lipomatous tumour/well-differentiated liposarcoma/MDM2/immunohistochemistry/fluorescence in situ hybridization分类
医药卫生引用本文复制引用
潘丹玲,陈小岩,陈灵锋,林瀛..非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤MDM2蛋白表达和基因扩增检测及临床意义[J].临床与实验病理学杂志,2017,33(7):759-762,4.
