临床与实验病理学杂志2017，Vol.33Issue(8)：858-862,5.DOI:10.13315/j.cnki.cjcep.2017.08.007
葡萄籽原花青素对糖尿病db/db小鼠肾组织细胞表型转化的影响
Effect of grape seed proanthocyanidins on phenotype marker protein of renal cells in db/db mice
摘要
Abstract
Purpose To investigate the effect of grape seed proanthocyanidins on the phenotype-transforming marker protein expression of db/db renal cells in mice model of type 2 diabetes,and to explore the protective mechanism of grape seed extract on diabetic renal injury in db/db mice.Methods Male db/db diabetic mice were randomly divided into two groups:diabetic group (db/db group) and diabetic + grape seed proanthocyanidin extract group (db/db + GSPE).The same week-old male db/m mice was used as normal controls (db/m) and grape seed proanthocyanidin extract gavage treatment group (db/m +grape seed proanthocyanidin extract group,db/m + GSPE).The mice of db/db + GSPE group and db/m + GSPE group were administered daily with grape seed proanthocyanidin extract (5mg/kg) by gavage.Results Renal tissues of db/db diabetic mice showed increased expression of α-SMA,p-p38MAPK,pERK1/2 and 8-OHdG level,and down-regulation in E-cadherin expression compared with db/m group (P ＜ 0.05).However,the alternations of α-SMA,p-p38,p-ERK1/2,E-cadherin protein levels,and 8-OHdG level,in db/db group were reversed by addition of grape seed proanthocyanidin extract (P ＜ 0.05).Conclusion Grape seed proanthocyanidin extract inhibits the epithelial to mesenchymal transition (EMT) associated protein,by decreasing ROS production,and activating p38 MAPK and ERK1/2.These findings suggest that grape seed proanthocyanidin extract provides a treatment option for diabetic nephropathy.关键词
糖尿病肾病/上皮-间质转化/葡萄籽原花青素/α-SMA/E-cadherinKey words
diabetic nephropathy/epithelial to mesenchymal transition/grape seed proanthocyanidin extract/α-SMA/E-cadherin分类
医药卫生引用本文复制引用
韦金英,史永红,任韫卓,杜云霞,杜春阳,段惠军,吴海江..葡萄籽原花青素对糖尿病db/db小鼠肾组织细胞表型转化的影响[J].临床与实验病理学杂志,2017,33(8):858-862,5.基金项目
河北省自然科学基金(H2016206482)、河北省高等学校科学技术研究项目(QN2015020) （H2016206482）
