临床与实验病理学杂志2017，Vol.33Issue(8)：863-867,5.DOI:10.13315/j.cnki.cjcep.2017.08.008
Necrostatin-1对输尿管梗阻小鼠肾脏炎症的影响
Effect of Necrostatin-1 on the inflammation in unilateral ureter obstruction mice
摘要
Abstract
Purpose To observe the effect of Necrostatin-1 (Nec-1),a necroptosis inhibitor,on the inflammation in unilateral ureter obstruction mice.Methods Male C57BL/6J mice were randomly divided into sham operation group,unilateral ureter obstruction operation group and UUO + Nec-1 treatment group,and the mice were sacrificed at 7th day after operated.Scr and BUN were measured.The pathological changes in the kidney were observed by HE staining.Immunohistochemistry and Western blot were used to detect the protein expression of necroptosis-related indicators RIP1,RIP3,MLKL and inflammatory cytokines TNF-α,IL-1β and MCP-1.Results Compared with sham operation group,the expression of RIP1,RIP3 and MLKL protein increased in the renal tissue of UUO mice,accompanied with increased expression of TNF-α,IL-1β and MCP-1.Nec-1 treatment significantly decreased above-mentioned protein expression in UUO mice,and also reduced renal interstitial inflammation and renal tubal injury according to HE staining.Scr and BUN levels suggested improved renal function.Conclusion Nec-1 could relieve the inflammatory reaction in renal tissue of the UUO mice by inhibiting necroptosis,which may be a new target for the treatment of secondary inflammation.关键词
炎症/输尿管梗阻/Necrostatin-1/necroptosisKey words
inflammation/ureteral obstruction/Necrostatin-1/necroptosis分类
医药卫生引用本文复制引用
肖夏,张涛,杨昕达,滕晴,侯佳,任韫卓..Necrostatin-1对输尿管梗阻小鼠肾脏炎症的影响[J].临床与实验病理学杂志,2017,33(8):863-867,5.基金项目
河北省首批青年拔尖人才项目(QNBJ16002)、河北省卫生厅重点科技研究计划(20150628)、河北省博士后科研资助项目(B2015003028) （QNBJ16002）
