临床与实验病理学杂志2017，Vol.33Issue(9)：959-963,5.DOI:10.13315/j.cnki.cjcep.2017.09.004
子宫颈癌中SIX1的表达及其与细胞增殖的关系
Expression of SIX1 in cervical cancer and relationship with cervical cancer cell proliferation
黄江梅 1肖芳 1陈艳昕 1李桃 1周颖 1赵敏 1张辉1
作者信息
摘要
Abstract
Purpose To investigate the expression of SIX1 in cervical cancer and its biological function.Methods The expression of SIX1 and Ki-67 in 35 cervical cancer tissues,42 cervical intraepithelial neoplasia (CIN) tissues and 15 normal cervical tissues was detected by immunohistochemical of EliVision two-step staining.A plasmid vector expressing SIX1 siRNA was constructed and then stably transfected into Hela cells.The expression of SIX1 mRNA and protein was examined by RT-PCR and Western blot method respectively.The proliferation was exanined by MTT.Results The positive rate of SIX1 in cervical cancer was significantly higher than that of CIN and normal cervical tissues and was positively correlation with the proliferation index of Ki-67.After RNAi treatment,the mRNA and protein levels of SIX1 was down-regulated in Hela cells and the proliferation of Hela cells was inhibited.Conclusion SIX1 may play an important role in the occunence and development process of cervical cancer by promoting cervical cancer cell proliferation.关键词
子宫颈肿瘤/子宫颈上皮内病变/SIX1/Ki-67Key words
cervical neoplasm/cervical intraepithelial neoplasia/SIX1/Ki-67分类
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黄江梅,肖芳,陈艳昕,李桃,周颖,赵敏,张辉..子宫颈癌中SIX1的表达及其与细胞增殖的关系[J].临床与实验病理学杂志,2017,33(9):959-963,5.
