林业科学2017，Vol.53Issue(10)：80-89,10.DOI:10.11707/j.1001-7488.20171009
基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建
Genetic Diversity and Fingerprints of Robinia pseudoacacia Clones Based on SSR Markers
摘要
Abstract
[Objective]To analyze genetic diversity and to construct the fingerprints of Robinia pseudoacacia clones selected in Shandong province,which can lay a solid foundation for R. pseudoacacia new varieties selection,breeding and identification,and also provide a scientific basis for the conservation,assessment and utilization of R. pseudoacacia in other provinces of China.[Method]Fluorescent SSR primers were used for PCR amplification. The products were detected with capillary electrophoresis. The results were used to analyze genetic diversity and to construct fingerprints of 49 R. pseudoacacia clones. [Result]A total of 51 alleles were identified using 8 pairs of SSR primers,with a mean of 6. 375 alleles per locus,ranging from 2 - 15. The mean value of PIC was 0. 5098,ranging from 0. 092 - 0. 879. Clustering analysis conducted with the method of average linkage between groups showed that clones'Luci 8'and 'Luci 13'from Linyi,'Luci 40'and 'Luci 42'from Qingdao,'Luci 10'and 'Luci 86'from Rizhao displayed the closest genetic relationship,respectively. The 49 clones were not clustered together strictly in accordance with geographic areas. There were no obvious correlations between grouping and current growing regions of the clones. A total of 9 primer pairs were used for clonal identification, two of them namely Rply109 and rops16 can distinguish 45 clones, indicating an identification rate of 91. 84% . Three alleles at one or two loci were detected in 22 clones,suggesting that these clones may be natural polyploidies.[Conclusion]R. pseudoacacia in Shandong province has relatively high genetic diversity. There were not obvious correlations between grouping and current growing regions of the clones. The two primers Rply109 and rops16 were determined to be efficient SSR markers for fingerprints construction and molecular identification of R. pseudoacacia clones,which can distinguish a proportion of 91. 84% of the total number of clones. The fingerprints constructed with SSR markers can provide a basis for germplasm resources management,variety identification and intellectual property rights protection,it also provides a scientific basis for introduction,genetic improvement and breeding of R. pseudoacacia.关键词
刺槐/SSR/分子标记/遗传多样性/指纹图谱/聚类分析Key words
Robinia pseudoacacia/SSR/molecular marker/genetic diversity/fingerprint/cluster analysis分类
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毛秀红,郑勇奇,孙百友,张元帅,韩丛聪,位晓,荀守华..基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建[J].林业科学,2017,53(10):80-89,10.基金项目
国家林业公益性行业专项"刺槐属种质资源收集保存与创新利用研究"(201304116) . （201304116）
