南京林业大学学报（自然科学版）2017，Vol.41Issue(6)：19-25,7.DOI:10.3969/j.issn.1000-2006.201703021
薄壳山核桃嫁接愈合过程中差异蛋白质的分析
Analysis of the differential proteins in the graft healing process of pecan (Carya illinoinensis)
摘要
Abstract
[Objective]Grafting is an important method for propagating pecan. This paper aimed to reveal the mechanism underlying graft healing on the proteomic level. [Method] We investigated the differential proteins in the graft union of pecans at four developmental stages (1, 6, 10 and 25 days after grafting) based on two-dimensional gel electrophoresis and MALDI-TOF/ TOF-MS. [Result] In total, 48 differential proteins were identified, and these identified proteins were classified into seven functional categories: energy metabolism, stress and defense responses, cell growth, secondary me-tabolism, protein synthesis, amino acid metabolism and function unknown. [ Conclusion ] Fructose-bisphosphate aldolase, phosphoglycerate kinase, pyruvate decarboxylase and adenosine triphosphatase could provide adequate energy for the graft healing of pecan. Ascorbate peroxidase and peroxiredoxins can eliminate the excessive reactive oxygen spe-cies caused by grafting. Soluble inorganic pyrophosphatase may play a role in promoting callus proliferation. Metacaspase and alpha tubulin may be associated with tracheary element differentiation.关键词
薄壳山核桃/嫁接/差异蛋白/双向电泳Key words
Carya illinoinensis/grafting/differentially expressed protein/two-dimensional electrophoresis分类
农业科技引用本文复制引用
莫正海,何海洋,陈文静,邓秋菊,彭方仁..薄壳山核桃嫁接愈合过程中差异蛋白质的分析[J].南京林业大学学报（自然科学版）,2017,41(6):19-25,7.基金项目
江苏省林业三新工程项目(Lysx[2016]44) （Lysx[2016]44）
国家林业局'948'项目(2015-4-16) （2015-4-16）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
