南京林业大学学报（自然科学版）2017，Vol.41Issue(6)：26-32,7.DOI:10.3969/j.issn.1000-2006.201610011
硫化氢对白桦悬浮细胞中多胺和次生代谢物积累的影响
The effect of H2S on the accumulation of polyamines and secondary metabolites in birch suspension cells
摘要
Abstract
[Objective]The purpose of this study was to clarify the effect of hydrogen sulfide (H2 S) on the accumulation of polyamines and secondary metabolites in birch suspension cultures. [Methods]Different concentrations of H2 S donor sodium hydrosulfide (NaHS) were added to a birch suspension culture. High performance liquid chromatography and colorimetric assays were used to analyze the levels of different forms of polyamines, enzyme activity of arginine decarbox-ylase (ADC) and polyamine oxidase (PAO), and the levels of flavonoids, polyphenols and triterpenoids. [Result] The total polyamine content of birch suspension cultures increased initially and then decreased after NaHS treatment. Among the cells, the highest level of putrescine and spermidine was at 1 mmol/ L NaHS after 24 h treatment, with increasing rates of 30.51% and 12.37% compared to the control, respectively. With the exception of perchloric acid (PCA)-insolu-ble spermidine conjugate, the content of free-, PCA-soluble and PCA-insoluble putrescines and conjugates increased af-ter NaHS treatment. Similarly, NaHS treatment enhanced the activity of ADC and PAO, with increasing ranges of 15. 91%-26. 33% and 137. 5%-250. 12%, respectively. Treatment on birch suspension cell with 1 mmol/ L NaHS and different concentrations of putrescine, spermidine, and spermine increased the polyphenol, flavonoid and triterpenoid levels, respectively. Polyphenol, flavonoid and triterpenoid reached maximum levels after combined treatment with 5 mmol/ L putrescine and NaHS; the levels increased by 26.69%, 13.22% and 4.55%, compared to NaHS treatment, re-spectively. [Conclusion] These results indicate that H2 S treatment increased the levels of polyamines and secondary me-tabolites, and the combined treatment with H2 S and polyamines further enhanced the accumulation of secondary metabo-lites in birch suspension cultures.关键词
悬浮细胞/硫化氢/多胺/次生代谢物Key words
birch (Betula platypylla Suk.)/suspension cells/hydrogen sulfide/polyamines/secondary metabolites分类
农业科技引用本文复制引用
王紫晴,李飞洁,郭玉婷,于昭慧,马钰斌,周文洋,范桂枝..硫化氢对白桦悬浮细胞中多胺和次生代谢物积累的影响[J].南京林业大学学报（自然科学版）,2017,41(6):26-32,7.基金项目
东北林业大学国家级大学生创新训练项目(201610225088) （201610225088）
黑龙江省自然科学基金项目(C2016005) （C2016005）
