南京林业大学学报（自然科学版）2017，Vol.41Issue(6)：41-46,6.DOI:10.3969/j.issn.1000-2006.201709011
茉莉酸甲酯对杂交鹅掌楸体胚发育的影响
Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid
摘要
Abstract
[Object] In order to further increase the frequency of somatic embryogenesis, the experiment was conducted to investigate the effect of methyl jasmonate ( MeJA) on somatic embryogenesis development of Liriodendron hybrid.[Methods]Based on an established system of somatic embryogenesis of Liriodendron hybrid, embryogenic cells of three genotypes included C138, 233012 and 253010 from Liriodendron chinense×L.tulipifera were cultured in the embryo in-duction medium with different concentrations of MeJA, and the rates of somatic embryogenesis, somatic embryo matura-tion, abnormal somatic embryos, and number of regeneration plants were investigated. [Result] The different concentra-tions of MeJA can significantly affect the efficiency of Liriodendron hybrid somatic embryogenesis, and with the increase of MeJA concentration, the rates of somatic embryogenesis and maturation gradually increased, the rate of abnormal em-bryos decreased gradually. However, when the MeJA concentration exceeds a certain value, the rates of somatic embryo-genesis and maturation showed a decreased trend, and the number of abnormal embryos also increased. Of which geno-type 233012 was in the first place as well as genotype C138 was higher than 253010. Abscisic acid (ABA) could en-hance the above-mentioned effects of MeJA. But the increasing concentration of MeJA restricted the root growth, and ABA could not alter this effect. [Conclusion]The induction effect of Liriodendron hybrid somatic embryos could be opti-mal when adding 1 mol/ L MeJA and 2 mg/ L ABA, the rates of somatic embryogenesis and somatic embryo maturation were relatively high, with the lowest rate of abnormal embryos.关键词
杂交鹅掌楸/体胚发生/茉莉酸甲酯/脱落酸Key words
Liriodendron chinense×L. tulipifera/somatic embryogenesis/methyl jasmonate/abscisic acid分类
农业科技引用本文复制引用
成铁龙,孟岩,陈金慧,施季森..茉莉酸甲酯对杂交鹅掌楸体胚发育的影响[J].南京林业大学学报（自然科学版）,2017,41(6):41-46,6.基金项目
国家高技术研究发展计划(2013AA102705) （2013AA102705）
教育部高等学校博士学科点专项科研基金项目(SRFDP20113204130002) （SRFDP20113204130002）
江苏省高校自然科学基金项目(13KJA220001) （13KJA220001）
江苏省高校青蓝工程项目(2015) （2015）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
