南京林业大学学报（自然科学版）2017，Vol.41Issue(6)：47-53,7.DOI:10.3969/j.issn.1000-2006.201512021
刺梨黄酮的半仿生法提取工艺及组分研究
Semi-bionic extraction of flavonoids from Rosa roxburghii Tratt.
摘要
Abstract
[Objective]The optimal conditions for the semi-bionic extraction ( SBE) of flavonoids from Rosa roxburghii Tratt. were identified. [Method]Response surface methodology ( RSM) was carried out to investigate the effects of etha-nol concentration, extraction temperature, solid-liquid ratio, and extraction time on the yield of flavonoids from Rosa roxburghii Tratt. [Result]The results showed that the optimal conditions for SBE were 80% ethanol, solid-to-liquid ratio of 1:13.2 ( mass volume ratio) , and extraction at 90℃ for 80-40-40 min;the extraction yield of flavonoids under these conditions was 27.70 mg/g. The antioxidant activity was significantly higher than that in the case of Tween auxiliary ex-traction and ultrasonic-assisted extraction. Component I and Component Ⅱ were separated by semi-preparing liquid chro-matography, and their retention times agreed with those of quercetin and kaempferol.[Conclusion]SBE is superior to the traditional ethanol extraction, and in combination with RSM, offers better yields of purified quercetin and kaempferol.关键词
刺梨/黄酮/半仿生法/响应面法Key words
Rosa roxburghii Tratt./flavonoid/semi-bionic extraction/response surface methodology分类
轻工纺织引用本文复制引用
张汇慧,刘龙云,吴彩娥,李婷婷,范龚健,应瑞峰..刺梨黄酮的半仿生法提取工艺及组分研究[J].南京林业大学学报（自然科学版）,2017,41(6):47-53,7.基金项目
江苏省科技支撑计划重点研发项目(BE2015315) （BE2015315）
江苏省自然科学基金项目(BK20150883) （BK20150883）
江苏省普通高校研究生科研创新计划项目(CXZZ120544) （CXZZ120544）
江苏省科技项目苏北科技专项(BN2016019) （BN2016019）
