南京林业大学学报（自然科学版）2017，Vol.41Issue(6)：177-180,4.DOI:10.3969/j.issn.1000-2006.201704060
小叶杨花粉母细胞减数分裂及花粉萌发力观测
Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr.
摘要
Abstract
[Objective]In order to lay the foundation for cross breeding and molecular cytogenetics of Populus simonii, the characteristics of the meiosis processes of microsporocytes and pollen germination ability were analyzed.[Method]We observed the meiosis processes of microsporocytes using carbol fuchin staining, and compared the pollen germination ca-pacity under different conditions for culturing flower branches.[Result]There was no obvious change in the flower buds except slightly thicker from the stage of microsporocyte to pollen grains during the meiosis. There was dynamic change of the number of nucleolus, lagged chromosomes and different kinds of spindles during the meiosis. The germination rate for the pollen grains of indoor flower branch is higher than the pollen grains of outdoor flower branch, and 2n gametes were observed from the pollen grains of outdoor flower branch. Different flower buds in one flowering branch or different florets in one flower bud showed non-synchronization in the meiosis processes of microsporocytes, but different anthers in one floret showed synchronization in the processes of meiosis. [Conclusion]The P. simonii could produce natural 2n pollen. It is an ideal species for the research of polyploid breeding and molecular cytogenetics of poplar.关键词
小叶杨/小孢子母细胞/减数分裂/2n花粉Key words
Populus simonii Carr./microsporocyte/meiosis/2n pollen分类
农业科技引用本文复制引用
兰月,辛昊阳,赵乙琏,孙志强,戴筱,解志伟,席梦利..小叶杨花粉母细胞减数分裂及花粉萌发力观测[J].南京林业大学学报（自然科学版）,2017,41(6):177-180,4.基金项目
江苏省高校自然科学研究重大项目(16KJA220001) （16KJA220001）
国家自然科学基金项目(31670603) （31670603）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
