南京林业大学学报（自然科学版）2017，Vol.41Issue(6)：187-192,6.DOI:10.3969/j.issn.1000-2006.201703027
低温胁迫下克恩氏冬青幼苗叶片差异蛋白质分析
Proteomic analysis of Ilex seedling leaf response to low temperature
摘要
Abstract
[Objective] Study the molecular mechanism of Ilex × koehneana ' Emily Bruner' in response to low temperature stress.[Mothed] We analyzed the proteomic differences in leaves among different temperature treatment (0℃ , -8 ℃ and -16 ℃ ) by using 2-DE coupled with MALDI-TOF/ TOF MS.[Result] The 2-DE profile of the total pro-tein in the leaves demonstrated that more than 1000 protein spots were detected with good reproducibility in the range of molecular masses of 15-120 ku and pH 4-7. A total of 31 protein spots in low temperature treatments exhibited signifi-cant changes in abundance of less than 0.66-fold or more than 1.5-fold (P<0.05) compared to CK. After MALDI-TOF/TOF MS analysis, a total of 23 differentially accumulated protein spots were successfully identified. Among the 23 identi-fied proteins, 20 proteins had assigned functions, and the other 3 were identified as novel with no assigned function. The 20 proteins with assigned functions were classified into 8 metabolic pathways and cellular processes, including protein and amino acid metabolism, carbohydrate metabolism, carbon fixation in photosynthetic organisms, lipid metabolism, photosynthesis, redox homeostasis, defense response, and nitrogen metabolism. The identifications of these differentially accumulated proteins indicates the presence of a specific different metabolic network in Ilex in response to cold stress.[Conclusions] These studies as a first step towards understanding the molecular mechanisms of low temperature resistance of Ilex, will provide the research basis for cultivating and screening relatively cold resistant varieties.关键词
克恩氏冬青/低温胁迫/差异蛋白质组/双向电泳Key words
Ilex×koehneana/low temperature stress/comparative proteomics/two-dimensional electrophoresis分类
农业科技引用本文复制引用
周余华,梁有旺,彭方仁..低温胁迫下克恩氏冬青幼苗叶片差异蛋白质分析[J].南京林业大学学报（自然科学版）,2017,41(6):187-192,6.基金项目
江苏省林业三新工程项目(Lysx[2016]44) （Lysx[2016]44）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
