南京医科大学学报（自然科学版）2017，Vol.37Issue(10)：1234-1238,5.DOI:10.7655/NYDXBNS20171002
噬菌体Guo1复苏结核休眠菌的初步研究
Resuscitation of mycobacteriophage Guo1 in dormant cells of M.tuberculosis
摘要
Abstract
Objective:To investigate the resuscitative potential of mycobacteriophage Guo1 on dormant cells of M.tuberculosis.Methods:Dormant cells of M.tuberculosis were induced by potassium-deficient culture of logarithmic phase of M.tuberculosis at 37 ℃,which were proven through drug sensitivity test,transmission electron microscope(TEM) and auramine O-Nile red double fluorescent staining.M.tuberculosis colony count was used to detect whether the dormant cells of M.tuberculosis had been resuscitated by Guo1.Results:Dormant cells of M.tuberculosis were obtained successfully by potassium-deficient cultivation for 30 days at 37 ℃.The concentration of M.tuberculosis in control,supernatant (SN),TM4 and Guo1 groups was (2.67±0.58)×102,(3.00±1.00)×102,(2.67±0.58)×102,(2.33±0.58)×102 CFU/mL,respectively,after culture for 1 d.And the concentration of M.tuberculosis in above groups increased to (7.33±1.53)×104,(6.67±2.08)×1010,(3.00±1.00)×1010,(3.33±1.53)×1010 CFU/mL at the 15th day,respectively.Conclusion:Mycobacteriophage Guo1 can resuscitate dormant cells of M.tuberculosis.关键词
噬菌体Guol/结核休眠菌/复苏/结核病Key words
bacteriophage Guo 1/dormant M.tuberculosis/resuscitation/tuberculosis分类
医药卫生引用本文复制引用
杨婷,杜丽娟,曹俊,郭述良..噬菌体Guo1复苏结核休眠菌的初步研究[J].南京医科大学学报（自然科学版）,2017,37(10):1234-1238,5.基金项目
国家自然科学基金面上项目(81570010) （81570010）
国家临床重点专科专项(2012-649) （2012-649）
