南京医科大学学报（自然科学版）2017，Vol.37Issue(10)：1257-1260,1277,5.DOI:10.7655/NYDXBNS20171006
基于硅纳米球探针构建的超灵敏电致化学发光免疫传感器用于丙肝核心抗原检测与临床诊断
Nanoparticle probe for the detection of HCV core antigen and clinical diagnosis
摘要
Abstract
Objective:To prepare Ru(bpy)32+(SiO2@Ru) coated silica nanospheres as electrochemiluminescence (electrogenerated chemiluminescence,ECL) biosensor probe,and evaluate its application value in the detection of the hepatitis C core antigen (HCVcAg).Methods:The water-in-oil(W/O) microemulsion method was employed for one-pot synthesis of SiO2@Ru nanoparticles.The as-synthesized SiO2@Ru nanoparticles has a narrow size distribution,which allowed reproducible loading of Ru (bpy)32+ inside the silica shell and HCVcAg,on the silica surface with glutaraldehyde as linkage.The as-synthesized SiO2@Ru could be used as a lable for ultrasensitive detection of HCVcAg through a sandwich immunoassay process.Results:The calibration range of HCVcAg concentration detection was 1-1 000 fmol/L,the lowest detection limit was 3 fmol/L.Compared with clinical RNA HCV,there was a good correlation,linear correlation coefficient was R2=0.937 9.Conclusion:With high sensitivity and good reproducibility,SiO2@Ru could be used as a label in clinical diagnosis,curative effect evaluation and blood screening of blood center.关键词
SiO2@Ru纳米复合物/HCVcAg/夹心法免疫检测/电致化学发光Key words
SiO2@Ru nanoparticles/HCVcAg/sandwich immunoassay/ECL分类
医药卫生引用本文复制引用
蔡心安,阮福明,郁金红,周镇先..基于硅纳米球探针构建的超灵敏电致化学发光免疫传感器用于丙肝核心抗原检测与临床诊断[J].南京医科大学学报（自然科学版）,2017,37(10):1257-1260,1277,5.基金项目
南京市科学技术厅社会发展项目(201201066) （201201066）
南京市医学科技发展项目(YKK15144) （YKK15144）
