南京医科大学学报（自然科学版）2017，Vol.37Issue(10)：1297-1301,5.DOI:10.7655/NYDXBNS20171015
锌转运体8自身抗体对1型糖尿病诊断价值及其与HLA-A基因的关系
Diagnostic value of zinc transporter 8 autoantibody to type 1 diabetes mellitus and its relationship with HLA-A gene
摘要
Abstract
Objective:To explore the diagnostic value of zinc transporter 8 autoantibody (ZnT8A) and the relationship between ZnT8A and human leukocyte antigen (HLA)-A genotype in the patients with type Ⅰ diabetes mellitus (T1DM) in Jiangsu Province.Methods:A cross-sectional and case-control study was carried out.The glutamic acid decarboxylase antibody (GADA),protein tyrosine phosphatase-2 antibody (IA-2A),insulin antibody (IAA),and ZnT8A were detected by radioimmunity ligand assay and the HLA gene polymorphism of HLA-A was examined with PCR-SSO method in T1DM and healthy controls (HC).Results:①Among 140 T1DM cases,46.4％ (65/140) had positive ZnT8A,while the positive rate in 102 healthy controls was 1.0％ (1/102).The positive rate of ZnT8A was significantly higher in T1DM than that in HC (P＜0.05).②On the base of testing GADA,IAA and IA-2A,the positive rate of at least one antibody was 70.7％(99/140) and it increasd to 86.4％ (121/140) when ZnT8A was further tested (P＜0.05).ZnT8A in the other three antibody-negative patients still had a positive rate of 15.7％.③HLA-A*24 in the T1DM group was significantly higher than that in the healthy control group (P＜0.05,OR=1.654),while A*33 decreased significantly (P＜0.05,OR=0.161) compared with the healthy control group.④HLA-A*02 was significantly higher in the ZnT8A-positive group than in the ZnT8A-negative group (P＜0.05,OR=0.318),but no significant difference was found after adjusting for the effect of course of disease,age of onset,and BMI.Conclusion:The positive rate of antibody increase by 15.7％ in T1DM patients when ZnT8A is further tested.ZnT8A can improve the diagnostic sensitivity.No relationship between HLA-A and ZnT8A was found.关键词
1型糖尿病/锌转运体8自身抗体/人类白细胞抗原Key words
type 1 diabetes/ZnT8A/HLA-A分类
医药卫生引用本文复制引用
钱莉,杨涛,陈小罗,王丽,吴晨光,徐志刚..锌转运体8自身抗体对1型糖尿病诊断价值及其与HLA-A基因的关系[J].南京医科大学学报（自然科学版）,2017,37(10):1297-1301,5.基金项目
镇江市社会发展科技支撑项目(SH2013052) （SH2013052）
