水产学报2017，Vol.41Issue(9)：1338-1344,7.DOI:10.11964/jfc.20160910560
核糖体DNA在厦门白姑鱼和大黄鱼染色体上的比较定位
Comparison of chromosome mapping of rDNA between Argyrosomus amoyensis and Larimichthys crocea
摘要
Abstract
Ribosomal DNAs were mapped comparatively with fluorescence in situ hybridization (FISH) in Argyrosomus amoyensis and Larimichthys crocea to explore the variations of karyotype microstructure among Sciaenids.The results showed that the karyotypes were quite different between A.amoyensis and L.crocea referring to macrokaryotype,and distribution mode of 18S rDNAs and 5S rDNAs.In A.amoyensis,the karyotype is 2n=48t (FN =48);single pair of 18S rDNA signals were mapped at interstitial region of Chromosome 1;and single pair of5S rDNA signals were located at proximal region of Chromosome 3.In L.crocea,the karyotype is 2n=2sm+4st+42t (FN =50);single pair of 18S rDNA signals were mapped at terminal region of short arm of Chromosome 18;and 9～11 pairs of 5S rDNA signals distributed mostly on centromeric termini of acrocentric chromosomes or terminal on the short arms.Together with the available cytogenetic data of Sciaenids,the following conclusions were drawn:(1) the karyotype of A.amoyensis represents characteristics of ancestral karyotype in Sciaenidae,while the karyoytpe of L.crocea was derived from the ancestral karyotype through rearrangement and transposition;(2) the macrokaryotype and the location of 18S rDNA sites were rather conservative in Sciaenidae with several exceptions,while the 5S rDNA sites varied dynamically among species.Hence,further studies to investigate microstructure variations of karyotypes among Sciaenid species with FISH should be encouraged.关键词
厦门白姑鱼/大黄鱼/染色体/荧光原位杂交/rDNAKey words
Argyrosomus amoyensis/Larimichthys crocea/chromosome/fluorescence in situ hybridization/rDNA分类
生物科学引用本文复制引用
廖梦香,郑娇,王志勇,张静,刘贤德,蔡明夷..核糖体DNA在厦门白姑鱼和大黄鱼染色体上的比较定位[J].水产学报,2017,41(9):1338-1344,7.基金项目
国家自然科学基金(31272653) （31272653）
福建省自然基金(2017J01449) （2017J01449）
全球变化与海气相互作用专项(GASI-02-SCS-YSWaut,GASI-02-SCS-YSWspr)National Natural Science Foundation of China (31272653) （GASI-02-SCS-YSWaut,GASI-02-SCS-YSWspr）
Natural Science Foundation of Fujian Province(2017J01449) （2017J01449）
Global Change and Air Sea Interaction (GASI-02-SCS-YSWaut,GASI-02-SCS-YSWspr) （GASI-02-SCS-YSWaut,GASI-02-SCS-YSWspr）
