水产学报2017，Vol.41Issue(9)：1400-1406,7.DOI:10.11964/jfc.20161110621
萱藻丝状体对无机碳的利用
Utilization of inorganic carbon in filaments of Scytosiphon lomentaria
摘要
Abstract
The utilization of inorganic carbon in filaments ofScytosiphon lomentaria was investigated by pH-drift technique and pH electrode method combined with related inhibitors.The influence of different concentrations of carbon dioxide on the activity of intracellular carbonic anhydrase (CA) was also explored at the same time.The results showed that (1)the pH compensation point of filaments of S.lomentaria was 9.21 and the CO2 compensation point was 0.74 iμmol/L.Acetazolamide (AZ) has no significant influence on the pH compensation and the inorganic carbon uptake amount in the first three hours of the inhibitor experiment.Ethoxyzolamide (EZ)and vanadate (Van) decreased the pH compensation point to the same value 8.82 and the inorganic carbon uptake amount inhibition rates were 55.93％ and 56.72％,respectively,in the first three hours of the inhibitor experiment.Under natural conditions,filaments of S.lomentaria use free CO2 and HCO3 as inorganic carbon source,and the utilization of HCO3-depends on ATPase and intracellular CA.(2) 0.35％,0.70％ and 1.20％ CO2 can inhibit the activity of intracellular CA of filaments of S.lomentaria and the inhibition rates were 6.29％,17.44％ and 27.15％,respectively.The higher CO2 concentration,the stronger inhibition effect of the activity of intracellular carbonic anhydrase.关键词
萱藻/丝状体/无机碳/pH/CO2/碳酸酐酶Key words
Scytosiphon lomentaria/filaments/inorganic carbon/pH/CO2/carbonic anhydrase分类
农业科技引用本文复制引用
王吉,宫相忠,高伟,罗伟,夏云捷,张红霞..萱藻丝状体对无机碳的利用[J].水产学报,2017,41(9):1400-1406,7.基金项目
山东省重点研发计划(2016GSF115042) （2016GSF115042）
国家“八六三”高技术研究发展计划(2012AA10A413)the Key Research and Development Program of Shandong Province (2016GSF115042) （2012AA10A413）
The National High Technology Research and Development Program of China (863 Program) (2012AA10A413) （863 Program）
