水产学报2017，Vol.41Issue(9)：1443-1454,12.DOI:10.11964/jfc.20160910554
山瑞鳖细菌性败血症病原菌的分离鉴定及其毒力基因检测
Isolation, identification and virulence genes detection of pathogenic bacteria from Palea steindachneri with bacterial septicemia
摘要
Abstract
In order to provide reference for effective prevention and control of the bacterial septicemia of Palea steindachneri,the present experiment was conducted to investigate the pathogen ofP.steindachneri with bacterial septicemia and the presence of six virulence genes.The pathogen was isolated from the heart and liver tissues of P.steindachneri by using conventional methods,and the pathogenicity of the strains was confirmed by artificial infection.Then the pathogen was identified based on API 20NE bacteria identification system and PCR amplification of 16S rRNA gene.Presence of virulence genes was determined using the polymerase chain reaction.Results showed that four dominant strains named SRB125,SRB142,SRB143 and SRB345 were isolated from the heart and liver tissues of diseased P.steindachneri.The mean fatality rate of healthy P.steindachneri was 97.50％-100.00％ and the pathogenic bacteria of bacterial septicemia of P.steindachneri were the four isolated strains.According to the biochemical and molecular identification results,the four isolated strains were all Aeromonas hydrophila,and they had the closest genetic relationship with A.hydrophila L3-5 (KP716701),sharing homology of 99.9％.The four strains contained two virulence genotypes,hly+Aer+Alt+Acfahal+ahp+ and hly+Aer+Alt+Acfahal+ahp-,each accounting for two strains.Strain SRB 143 from Narming City and SRB345 from Gulping City all lacked gene ahp.关键词
山瑞鳖/细菌性败血症/嗜水气单胞菌/毒力基因Key words
Palea steindachneri/bacterial septicemia/Aeromonas hydrophila/virulence gene分类
农业科技引用本文复制引用
韩书煜,梁静真,覃志彪,黄艳华,韦慕兰,蒙兰丽,黄维,胡大胜,黄钧..山瑞鳖细菌性败血症病原菌的分离鉴定及其毒力基因检测[J].水产学报,2017,41(9):1443-1454,12.基金项目
广西水产畜牧兽医局专项(桂渔牧财[2013135号,桂渔牧财[2014152号,桂渔牧财[2015197号) （桂渔牧财[2013135号,桂渔牧财[2014152号,桂渔牧财[2015197号）
南宁市科技局科技计划项目(南科发(2012) 51号-20122096 （南科发(2012）
南科发(2013) 46号-20132124)Project of Bureau of Fishery Animal Husbandry and Veterinary,Guangxi Province (Gui-yu-mu-cai [2013] No.35,Gui-yu-mu-cai [2014] No.52,Gui-yu-mu-cai [2015] No.97) （2013）
Project of Science and Technology Plan of Bureau of Nanning Science and Technology (Nan-ke-fa [2012] No.51-20122096,Nan-ke-fa [2013] No.46-20132124) （Nan-ke-fa [2012] No.51-20122096,Nan-ke-fa [2013] No.46-20132124）
