水产学报2017，Vol.41Issue(11)：1660-1666,7.DOI:10.11964/jfc.20160810523
环境污染物对斑马鱼早期胚胎转座子转录活性的影响
Effects of environmental pollutants on transcription activity of transposons during the early embryonic development of zebrafish (Danio rerio)
摘要
Abstract
Transposons are movable and extended elements, they are important parts in genome and can affect the genome evolution.Transposable activity is usually regulated by environmental factors.To discuss the influence of environmental stress on the activity of transposons, the zebrafish embryos at early stage were exposed to TCDD, Cu2+ and Cd2+ for two hours, respectively.Then the embryos were cultured in normal medium until 72 hpf.The total RNA of embryos were extracted and transcribed into cDNA by reverse transcription.Then the transcription activity of 9 transposons with complete gene structures after exposure were compared with that before by real-time quantity fluorescent PCR.The 9 transposons included 5 DNA transposons (Tc-a, Tc-b, Tc-c, Tc-d and Tc-e) and 4 retrotransposons (ZB-ERV-1, ZB-ERV-2, 2 L1-323 and L1-21).The results showed that the transcription activity of 8 transposons treated with TCDD obviously decreased, and that of one significantly increased; the transcription activity of 7 transposons treated with Cu2+ obviously increased, and that of 2 were significantly decreased; the transcription activity of 6 transposons treated with Cd2+ obviously increased, and that of 3 significantly decreased.Those results suggested that environment factors can significantly affect transposons expression activity, and environment stress could be the important reason for the variation of transposon activity.Those data play a very important role in evaluating and understanding the effect of environment factors on fish genome and transposon activity, and provide reference for further research on biology genome evolutionary mechanism.关键词
斑马鱼/转座子/污染物/转录活性Key words
Danio rerio/transposon/environment pollutants/transcription activity分类
生物科学引用本文复制引用
高波,陈才,张丽,沈丹,王赛赛,陈伟,王伟,产舒恒,宋成义..环境污染物对斑马鱼早期胚胎转座子转录活性的影响[J].水产学报,2017,41(11):1660-1666,7.基金项目
国家自然科学基金(31671313) （31671313）
江苏省优势学科专项 Natural Science Foundation of China (31671313) （31671313）
Priority Academic Program Development of Jiangsu Higher Education Institutions （）
