水产学报2017，Vol.41Issue(11)：1667-1679,13.DOI:10.11964/jfc.20161010568
正常与性早熟中华绒螯蟹的Y-器官转录组分析
Transcriptome sequencing and functional analysis of Y-organs between normal and precocious Chinese mitten crabs (Eriocheir sinensis)
摘要
Abstract
Precocity is a common problem in aquaculture of Chinese mitten crab (Eriocheir sinensis).To discover and clone important functional genes, the transcriptome of Y-organs of precocious and normal female crabs were sequenced by Illumina Hiseq 2000 high-throughput sequencing technology, and comparative transcriptome study was conducted.The results showed that precocious and normal crabs had 44619538 and 43052958 clean reads, respectively.Comparison between the sequencing data from precocious and normal female crabs revealed 2655 differentially expressed genes.The differentially expressed genes GO functions in the transcriptome library were broadly divided into three major ontology (biological processes, cellular component and molecular function categories) of 42 branches.Data in the transcriptome from 134 differentially expressed genes could be divided into 4 classes taking the KEGG database as a reference, according to the metabolic pathway, including synthesis and degradation of ketone bodies, butanoate metabolism, neurotrophin signaling pathway, and base excision repair.By RNA-seq, we obtained abundant transcriptome information that could contribute to novel gene identification, causes and prevention of precocity, and research of Y-organs in Eriocheir sinensis.关键词
中华绒螯蟹/性早熟/Y-器官/转录组Key words
Eriocheir sinensis/precocity/Y-organ/transcriptome分类
生物科学引用本文复制引用
张佳鑫,徐敏杰,黄根勇,张聪,杨志刚,成永旭,杨筱珍..正常与性早熟中华绒螯蟹的Y-器官转录组分析[J].水产学报,2017,41(11):1667-1679,13.基金项目
国家自然科学资金(31272677 （）
31472287) （）
上海市科技兴农推广项目[沪农科推字(2015)第1-7号] （2015）
上海市科委项目(16DZ2281200) （16DZ2281200）
现代农业产业技术体系专项 National Natural Science Fund of China (31272677 （）
31472287) （）
Shanghai Agricultural Commission (2015D1-7) （2015D1-7）
Shanghai Science and Technology Commission (16DZ2281200) （16DZ2281200）
China Agriculture Research System （）
