马氏珠母贝TLR6基因的克隆、序列分析与表达OA北大核心CSCDCSTPCD
Cloning, sequence analysis and expression studies on PmTLR6 gene from Pinctada fucata martensii
为了探究TLR6(Toll like receptor 6)在马氏珠母贝免疫反应中的作用,实验采用cDNA末端快速扩增(RACE)技术获得了马氏珠母贝TLR6基因(Pm-TLR6)cDNA全长序列,并且运用实时荧光定量PCR(qRT-PCR)技术检测了Pm-TLR6在马氏珠母贝各组织中的表达情况、哈维氏弧菌刺激后和植核移植后血淋巴中的表达模式.结果显示:Pm-TLR6 cDNA全长2295 bp,其中5′非编码区(UTR)长94 bp,3′UTR…查看全部>>
TLR6 (Toll like receptor 6) is a kind of pattern recognition receptors and plays an important role in resisting microorganism infection.To study the function of TLR6 in the immune response of P.fucata martensii, in this study, a full length ofPm-TLR6 was obtained using rapid amplification of cDNA ends (RACE) technology from P.fucata martensii.The expression patterns ofPm-TLR6 in all tissues and its sequential expression in the hemolymph after Vibrio harveyi …查看全部>>
吴羽媛;郭志颖;梁海鹰;林丽旋;郝瑞娟;焦钰
广东海洋大学水产学院,广东湛江 524025广东省珍珠养殖与加工工程技术研究中心,广东湛江 524025广东海洋大学水产学院,广东湛江 524025广东海洋大学水产学院,广东湛江 524025广东省珍珠养殖与加工工程技术研究中心,广东湛江 524025广东海洋大学水产学院,广东湛江 524025
生物科学
马氏珠母贝TLR6基因克隆表达分析
Pinctada fucata martensiiTLR6gene cloningexpression analysis
《水产学报》 2017 (11)
马氏珠母贝植核育珠移植耐受免疫相关基因与蛋白质鉴定及关联度研究
1687-1698,12
国家自然科学基金(31472306)广东省科技计划项目(2012A031100010)广东省海港建设与渔业产业发展专项(A201608B15)国家级大学生创新创业训练项目(201410566005)广东海洋大学大学生创新创业训练项目(CXXL2014012) National Natural Science Foundation of China (31472306) Science and Technology Plan Projects of Guangdong Province, China (2012A031100010) Harbor Construction and Development of Special Fisheries Industry of Guangdong Province, China (A201608B15) National College Students' Innovation Entrepreneurship Training Program (201410566005) College Students' Innovation Entrepreneurship Training Projects of Guangdong Ocean University (CXXL2014012)
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