水产学报2017，Vol.41Issue(11)：1699-1709,11.DOI:10.11964/jfc.20161010598
莫荷罗非鱼"广福1号"与其亲本间DNA甲基化的差异分析
Analysis of DNA methylation difference between "Mohe" hybrid tilapia and its parents
摘要
Abstract
DNA methylation difference of eleven tissues of Oreochromis mossambicus,Oreochromis hornorum and " Mohe" hybrid tilapia (O.mossambicus♀×O.hornorum♂), such as skin, muscle and gill etc.was analyzed to explore the change of methylation pattern during the interspecific hybridization using methylation sensitive amplified polymorphism (MSAP).16 pairs of primers were used to amplify selectively.The results of electrophoretic banding analysis showed that there were differences in the methylation levels among these different tissues of the same species and between the same tissues among " Mohe" hybrid tilapia and its parents; the average methylation level of these tissues from O.mossambicus,O.hornorum and " Mohe" hybrid progenies was 32.21%, 38.03% and 29.77%, respectively.The methylation level of hybrid progenies was lower than those of the parental progenies.Most of the methylation patterns of the hybrid progenies were similar to the parental progenies (A, B and C types, 19.67%, 29.99% and 25.42%), while 24.92% cytosine demethylation and hypermethylation were found in the genome of hybrid progenies and the number of demethylation sites (E type, 15.73%) was greater than that of hypermethylation sites (D type, 9.19%).These results suggested that the genomic DNA hypomethylation and the reshuffling of the DNA methylation pattern in the hybrid progenies might be associated with the heterosis.The present findings established epigenetic foundation to further reveal the heterosis mechanism of tilapia.关键词
莫荷罗非鱼/杂交亲本/杂种优势/DNA甲基化/甲基化敏感扩增多态技术Key words
" Mohe" hybrid tilapia/hybrid parents/heterosis/DNA methylation/MSAP分类
生物科学引用本文复制引用
尚慧文,刘志刚,朱华平,高风英,可小丽,卢迈新..莫荷罗非鱼"广福1号"与其亲本间DNA甲基化的差异分析[J].水产学报,2017,41(11):1699-1709,11.基金项目
中国水产科学研究院基本科研业务费(2016HY-ZC0401) （2016HY-ZC0401）
现代农业产业技术体系专项(CARS-46) （CARS-46）
国家科技支撑计划子课题(2012BAD26B03) Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2016HY-ZC0401) （2012BAD26B03）
China Agriculture Research System (CARS-46) （CARS-46）
National Key Technology R&D Program (2012BAD26B03) （2012BAD26B03）
